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2.3. Secondary Screening of Active Isolate and Antifungal Activity The isolate PM0525875 which exhibited exclusive antifungal activity was screened for its reproducibility as per the procedure mentioned in 2.2. The methanolic extract of the whole culture broth, cell mass extract and cell free culture filtrate were tested against Candida sp. to confirm the antifungal activity. To rule out the presence of polyene in the active extract, the UV-visible spectrum of extract was compared with the UV-visible spectrum of standard polyenes classes from the literature [15]. |
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2.3. 活性分離物及其抗真菌活性的二次篩選 用2.2所提及的過程對具有獨特抗真菌活性的分離物PM0525875進(jìn)行篩選,以確定期重復(fù)性。用全培養(yǎng)液和細(xì)胞團的甲醇提取物,以及無細(xì)胞培養(yǎng)的濾液液對念珠菌屬進(jìn)行了測試以確定其抗真菌活性。為了排除活性提取物中存在多烯類,紫外 - 可見光光譜...... |
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