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3.2. Large Scale Fermentation of PM0525875-V4 Large scale fermentation was carried out using PM0525875-V4 and fermentation medium ASW-36P(1). The fermentation was carried out till 96 h. The time course of fermentation batch is shown in Table 4 and expressed in graphical form as shown in Figure 2. During fermentation, the pH was dropped with the time and later main- tained to 6.5 till end of the fermentation. The rise in biomass (PCV) was slow initially and was picked up after 24 h. After 48 h, the growth rate was steady and the stationary phase was achieved. Dissolve oxygen (DO)dropped significantly till 24 h and was maintained at around 40% till end of the process. The total sugar consumption was slow at beginning and it was reduced to half of the concentration at 48 h. Based on the bioactivity pattern obtained by the broth dilution method (as mentioned in 2.4), the maximum activity was attended at 72 hand maintained steady till end of the fermentation process. Hence, the fermentation cycle was terminated at 96h. |
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【答案】應(yīng)助回帖
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3.2. Large Scale Fermentation of PM0525875-V4 Large scale fermentation was carried out using PM0525875-V4 and fermentation medium ASW-36P(1). The fermentation was carried out till 96 h. The time course of fermentation batch is shown in Table 4 and expressed in graphical form as shown in Figure 2. During fermentation, the pH was dropped with the time and later main- tained to 6.5 till end of the fermentation. 3.2 PM0525875-V4的大規(guī)模的發(fā)酵 使用PM0525875-V4和發(fā)酵媒介ASW-36P(1)進(jìn)行大規(guī)模的發(fā)酵，發(fā)酵時(shí)間為96小時(shí)，發(fā)酵批次的時(shí)間表如表4和圖2所示，在發(fā)酵期間，PH值隨著時(shí)間的增長(zhǎng)而降低，直到發(fā)酵的最后階段，保持Ph值為6.5 |
2樓2014-10-26 16:54:22
蹉跎歲月
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- 翻譯EPI: 40
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- 蟲號(hào): 2021440
- 注冊(cè): 2012-09-22
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
bxzc123: 金幣+30, 翻譯EPI+1, ★★★很有幫助 2014-10-26 21:25:39
bxzc123: 金幣+30, 翻譯EPI+1, ★★★很有幫助 2014-10-26 21:25:39
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生物量的增長(zhǎng)在起初緩慢，24小時(shí)后將其收集起來 48小時(shí)后，生長(zhǎng)速率平穩(wěn)，并達(dá)到穩(wěn)定期 直到24小時(shí)，溶解氧開始顯著降低，并最后階段保持在40%左右， 全部糖的消耗在剛開始少，在48小時(shí)的時(shí)候，其濃度減少到一半。 根據(jù)稀釋法獲得的生物活性模式（在2.4提及），最大的活性達(dá)到72,并一直平穩(wěn)的保持到整個(gè)發(fā)酵過程的結(jié)束，因此一個(gè)發(fā)酵周期為96小時(shí)。 |
3樓2014-10-26 17:05:34
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