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Cytogenetic Analysis of Asiatic Lily Cultivars and Their Hybrids Using Fluorescence In Situ Hybridization 作者:Gao, T (Gao, T.)[ 1 ] ; Sun, H (Sun, H.)[ 1 ] ; Fang, L (Fang, L.)[ 1 ] ; Qian, H (Qian, H.)[ 1 ] ; Xin, H (Xin, H.)[ 1 ] ; Shi, J (Shi, J.)[ 1 ] ; Wu, Z (Wu, Z.)[ 1 ] ; Xi, M (Xi, M.)[ 1 ] 編者:VanTuyl, JM III INTERNATIONAL SYMPOSIUM ON THE GENUS LILIUM 叢書: Acta Horticulturae 卷: 1027 頁(yè): 177-184 出版年: 2014 會(huì)議名稱 會(huì)議: 3rd International Symposium on the Genus Lilium 會(huì)議地點(diǎn): Zhangzhou, PEOPLES R CHINA 會(huì)議日期: APR 01-03, 2014 會(huì)議贊助商:Int Soc Hort Sci 摘要 Two Asiatic lily cultivars, 'Golden Horn', 'Pollyanna', and their six progenies were analyzed using Fluorescence in situ hybridization (FISH) with 45S rDNA as probe. It was shown that five progenies were diploid (2n=2x=24), one progeny was triploid (2n=3x=36), We observed a total of 11 hybridization signals on chromosome No 1, 2, 3, 4, 7 and 11 in 'Golden Horn', 10 hybridization signals occurred on chromosome No 1, 2, 4, 6, 7 and 11 in 'Pollyanna'. 45S rDNA loci were variable on chromosome No 3 and 6 of these two cultivars. Chromosome No 3 in 'Golden Horn' contained two 45S rDNA loci which were observed at the centromere and long arm, while 45S rDNA locus on chromosome No 6 in 'Pollyanna' was on the long arm. In addition, 45S rDNA loci on chromosome No 1 and 7 were located near the centromere, on chromosome No 2 the signals were near the secondary constriction and on chromosome No 4 and 11 were on the long arm. There were either 9 or 11 45S rDNA loci in the five diploid hybrids, and there were 15 loci in the triploid progeny. FISH detection of 45S rDNA clearly showed that all 45S rDNA signals in four progenies (GHP16, GHP142, GHP223, GHP245) were coming from 'Golden Horn' and 'Pollyanna', respectively. 關(guān)鍵詞 作者關(guān)鍵詞:Lilium; hybrids; fluorescence in situ hybridization; 2n gametes KeyWords Plus:SEXUAL POLYPLOIDIZATION; LILIUM; INTROGRESSION; MECHANISMS; PLANTS; GISH 作者信息 通訊作者地址: Xi, M (通訊作者) 顯示增強(qiáng)組織信息的名稱 Nanjing Forestry Univ, Minist Educ, Key Lab Forest Genet & Biotechnol, Nanjing 210037, Jiangsu, Peoples R China. 地址: 顯示增強(qiáng)組織信息的名稱 [ 1 ] Nanjing Forestry Univ, Minist Educ, Key Lab Forest Genet & Biotechnol, Nanjing 210037, Jiangsu, Peoples R China 電子郵件地址:ximenglinjfu@126.com 出版商 INT SOC HORTICULTURAL SCIENCE, PO BOX 500, 3001 LEUVEN 1, BELGIUM 類別 / 分類 研究方向  lant Sciences; AgricultureWeb of Science 類別 lant Sciences; Horticulture文獻(xiàn)信息 文獻(xiàn)類型 roceedings Paper語(yǔ)種:English 入藏號(hào): WOS:000343865600019 ISBN:978-94-62610-05-7 ISSN: 0567-7572 其他信息 IDS 號(hào): BB5GX Web of Science 核心合集中的 "引用的參考文獻(xiàn)": 20 Web of Science 核心合集中的 "被引頻次": 0 |
5樓2014-12-02 10:32:23
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