版塊導(dǎo)航: 正在加載中...

應(yīng)《網(wǎng)絡(luò)安全法》要求，自2017年10月1日起，未進(jìn)行實(shí)名認(rèn)證將不得使用互聯(lián)網(wǎng)跟帖服務(wù)。為保障您的帳號(hào)能夠正常使用，請(qǐng)盡快對(duì)帳號(hào)進(jìn)行手機(jī)號(hào)驗(yàn)證，感謝您的理解與支持！

24小時(shí)熱門版塊排行榜

返回列表

本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看

bxzc123

金蟲 (正式寫手)

應(yīng)助: 0 (幼兒園)
金幣: 40.9
散金: 503
紅花: 1
帖子: 344
在線: 62.4小時(shí)
蟲號(hào): 2384387
注冊(cè): 2013-03-28
專業(yè): 微生物生理與生物化學(xué)

[求助] 求翻譯，謝謝，站內(nèi)信求大神幫忙

A novel glyA gene from the marine bacterium Alcanivorax sp. was cloned and expressed in Escherichia coli BL21(DE3). The recombinant glyA encodes a polypeptide of 418 amino acids, which was designated as AdSHMT that shows the highest identity (70%) with a SHMT from Shewanella algae. The purified enzyme showed
a single band at about 45 kDa by SDS-PAGE analysis. It was found that AdSHMT exhibited the maximal activity at 50 ◦ C and pH 7.0. The K m , V max , and K cat values of AdSHMT against dl-threo-3-phenylserine were calculated to be 0.097 mol/L, 3.255 ?mol/min/mg and 2.451/s, respectively. More importantly, RP-HPLC detection showed that the AdSHMT achieved an 88.37% molecular conversion rate in catalyzing glycine to l-serine, with the final concentration of l-serine being 353.15 mM in the reaction at 35 ◦ C and 22nd hour when the initial concentration of the substrate (glycine) was 0.399 M. The molecular conversion rate of the AdSHMT from the Alcanivorax sp. was 1.26-fold that of the EcSHMT from the E. coli,which is currently applied in industrial production. Therefore, AdSHMT has the potential for industrial applications due to its high enzymatic conversion rate.

回復(fù)此樓

» 猜你喜歡

278求調(diào)劑已經(jīng)有9人回復(fù)
297求調(diào)劑已經(jīng)有12人回復(fù)
0703化學(xué)297求調(diào)劑已經(jīng)有3人回復(fù)
材料學(xué)碩301分求調(diào)劑已經(jīng)有6人回復(fù)
280求調(diào)劑已經(jīng)有11人回復(fù)
311求調(diào)劑已經(jīng)有3人回復(fù)
求調(diào)劑已經(jīng)有3人回復(fù)
313求調(diào)劑已經(jīng)有4人回復(fù)
317求調(diào)劑已經(jīng)有15人回復(fù)
307求調(diào)劑已經(jīng)有3人回復(fù)

1樓 2014-11-24 12:15:49

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

UTKProgress

木蟲 (小有名氣)

翻譯EPI: 11
應(yīng)助: 7 (幼兒園)
金幣: 3605.5
散金: 7
紅花: 3
帖子: 280
在線: 236.4小時(shí)
蟲號(hào): 3500502
注冊(cè): 2014-10-27
性別: GG
專業(yè): 高分子合成化學(xué)

【答案】應(yīng)助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
bxzc123: 金幣+20, 翻譯EPI+1, ★★★很有幫助 2014-11-25 22:11:57

一種來自海洋細(xì)菌Alcanivorax的新型的glyA基因被克隆，并在大腸桿菌BL21（DE3）上表達(dá)。重組的glyA 編碼了一個(gè)由418個(gè)氨基酸組成的多肽，被命名為 AdSHMT，它顯示出與來自Shewanella藻的SHMT最高70％的相似。在SDS-PAGE分析中純化的酶為45 kDa的單鏈 (多肽)。結(jié)果發(fā)現(xiàn)，在50◦C和pH7.0時(shí)，AdSHMT表現(xiàn)出最大的活性。AdSHMT against dl-threo-3-phenylserine (這里的一個(gè)專業(yè)知識(shí)點(diǎn)，我不懂。應(yīng)該是一生化反應(yīng))的 Km, Vmax, 和 Kcat 數(shù)值分別為0097摩爾/升，3255摩爾/分鐘/毫克和2451/秒。更重要的是反相高效液相色譜法檢測(cè)表明，在35 ◦ C時(shí)（AdSHMT）催化glycine為l-serine反應(yīng)中當(dāng)glycine的初始濃度為0.399 M時(shí)在第22小時(shí)AdSHMT取得了88.37%的轉(zhuǎn)化率。從Alcanivorax SP到AdSHMT的分子轉(zhuǎn)化率是目前在工業(yè)生產(chǎn)中應(yīng)用的從E. coli到EcSHMT的轉(zhuǎn)化率的1.26倍。因此，AdSHMT因其高的酶轉(zhuǎn)化率可能在工業(yè)上得到應(yīng)用。

贊一下(2人)

回復(fù)此樓

Letmeknowifyouneedanyhelponyourpaper.

2樓2014-11-25 10:06:15

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

相關(guān)版塊跳轉(zhuǎn) 我要訂閱樓主 bxzc123 的主題更新