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| 精密稱取各多糖樣品約15 mg，置于10 mL具塞試管中，加蒸餾水1 mL，加熱溶解，再加入2 mL，2 mol•L-1三氟乙酸溶液，搖勻，密封。將其放入烘箱中，110℃加熱水解8 h，取出冷卻至室溫后，室溫下離心5 min，轉(zhuǎn)速12000 r•min-1，移出上清液至10 mL容量瓶中，用2 mol•L-1 NaOH中和至PH約7.0，水定容至刻度，搖勻，PMP衍生化，含量測(cè)定。色譜柱：迪馬科技PLATISIL ODS柱（250 mm × 4.6 mm，5 μm）；柱溫：35℃；流動(dòng)相：乙腈-20 mmol•L-1醋酸銨緩沖液（22∶78）（PH=5.5）；流速：1.0 mL•min-1；紫外檢測(cè)波長(zhǎng)：245 nm；進(jìn)樣量：10 μL。 |
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木蟲(chóng)之王 (文學(xué)泰斗)
- 翻譯EPI: 31
- 應(yīng)助: 272 (大學(xué)生)
- 金幣: 70385.5
- 散金: 5156
- 紅花: 104
- 沙發(fā): 17
- 帖子: 126010
- 在線: 2961.6小時(shí)
- 蟲(chóng)號(hào): 2406939
- 注冊(cè): 2013-04-08
- 專業(yè): 聚合物共混與復(fù)合材料
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
80后的末班車: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 2014-12-01 20:05:50
80后的末班車: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 2014-12-01 20:05:50
| A precise quantity (~15 mg) of polysaccharide sample was weighed and put in a 10 mL test tube with a plug, to which 1 mL distilled water was added. The test tube was heated such that the polysaccharide sample was dissolved, and 2 mL trifluoroacetic acid (2 mol•L-1) was added. The mixed solution was shaken uniformly and sealed. The test tube was then placed in an oven, where it was heated at 110 ℃ for hydrolysis. After 8 h it was taken out, cooled to ambient temperature, and centrifuged at 12000 r•min-1 for 5 min. The supernatant was collected and transferred to a 10 mL volumetric flask, where it was neutralized by NaOH (2 mol•L-1) to pH = ~7.0, diluted with water to volume, and shaken uniformly for PMP derivation and content measurement. The chromatographic column is a PLATISIL ODS column (250 mm × 4.6 mm, 5 μm) made by Dikma Technologies Inc. The column temperature is 35℃. The mobile phase is acetonitrile - 20 mmol•L-1 ammonium acetate buffer（22∶78） with pH = 5.5. The flow rate is 1.0 mL•min-1. The UV detection wavelength is 245 nm. The injection volume is 10 μL. |
2樓2014-12-01 19:41:37
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