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3.8. Viability of cells cultured on PLGA/PHBV scaffolds Viability of cells cultured on PLGA/PHBV scaffolds was evaluated by means of kinetic examinations and observation of hMSCs, which was quantified by MTT and Live/Dead assay respectively. As shown in Fig. 8, cell viability of the cells on each group of scaffolds increased gradually when the culture days went longer. However,there was no significant differentiation between the cell viability of all groups on day1, day 3 and day 7. The result indicated that the adding of PHBV microspheres had no significant influence on the cell viability. The viability of hMSCs on the scaffolds was generally visualized using fluorescent Live/Dead assay. Fig. 9 indicated the cells status at day 7 and 14 after being seeded on three kinds of scaffold. On day 7, few dead cells (red spots) can be observed, which indicated that at the first few days there were several dead cells with damaged membranes [35]. With the increase of incubation time, more and more green spots can be observed which indicated that the cell proliferated well on the scaffolds. Besides, some graphs showed that the green light formed several circles which indicated that the cells were attached on the microspheres, which was coincident with the results of SEM micrographs. |
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2樓2014-12-08 08:58:23
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培養(yǎng)在PLGA/PHBV支架上的細(xì)胞的活性測(cè)試 培養(yǎng)在PLGA/PHBV支架上的細(xì)胞的活性借助于動(dòng)力學(xué)檢查（MTT測(cè)試）和對(duì)hMSCs的觀察（活/死法）進(jìn)行評(píng)估。如圖8所示，每組支架上的細(xì)胞的活性隨著培養(yǎng)天數(shù)的增長(zhǎng)而增加。然而，所有組的細(xì)胞活性在第1、3、7天沒(méi)有明顯差異。這一結(jié)果表明，PHBV微球的加入對(duì)細(xì)胞活性沒(méi)有明顯影響。hMSCs在支架上的活性通常通過(guò)熒光活/死法進(jìn)行可視化檢測(cè)。圖9顯示種在3中支架上的細(xì)胞在第7天和第14天的狀態(tài)。在第7天時(shí)，能觀察到少許死細(xì)胞（紅色的點(diǎn)），這表明在最初的幾天有少數(shù)細(xì)胞隨細(xì)胞膜破壞而死亡。隨著孵育時(shí)間的延長(zhǎng)，可觀察到越來(lái)越多的綠色的點(diǎn)，表明細(xì)胞能在支架上很好地增殖。此外，一些圖中出現(xiàn)了綠光形成的圓圈，表明細(xì)胞粘是附在微球上的，這與SEM現(xiàn)實(shí)的結(jié)果一致。 |
3樓2014-12-08 16:49:39
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