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Nitrogen compounds such as nitrate act as a potential inhibitor for legume nodulation. In this study, we isolated a new CLE gene, GmNIC2, from nitrate-treated roots, which shares high sequence homology with nitrate-induced CLE gene GmNIC1. Similar to GmNIC1, the expression level of GmNIC2 was not significantly altered in roots by rhizobial inoculation and was much higher in young nodules than in roots. In addition, overexpression of GmNIC2 led to similar nodulation inhibition of transgenic hairy roots to that of GmNIC1, which occurred in GmNARK-dependent manner and at the local level. By analyzing GmNARK loss-of-function mutant, SS2-2, it was found that expression levels of GmNIC1 and GmNIC2 in the SS2-2 roots were lower than in the wild type (WT) roots in response to nitrate. In contrast to GmNIC1 and GmNIC2, expressions of GmRIC1 and GmRIC2 genes that are related to the autoregulation of nodulation (AON) were strongly suppressed both of the soybeans during all periods of nitrate treatment and even were not induced by additional inoculation with rhizobia. Taken together, the results of this study suggest that GmNIC2, as an active homologous gene located in chromosome 13, acts locally to suppress nodulation, like GmNIC1, and nitrate inhibition of nodulation is led by fine-tuned regulation of both nitrate-induced CLEs and rhizobia-induced CLEs. Abbreviations AON, autoregulation of nodulation;CaMV, cauliflower mosaic virus;cDNA, complementary DNA;CLE, CLAVATA3/ESR-related;DAI, days after inoculation; GFP, green fluorescent protein；GmENOD40, Glycine max EARLY NODULIN 40; GmNARK, Glycine max NODULE AUTOREGULATION RECEPTOR KINASE; GmRIC, Glycine max RHIZOBIUM INDUCED CLE; GmNIC, Glycine max NITRATE INDUCED CLE; GUS, β-glucuronidase; HAR1, HYPERNODULATION ABERRANT ROOT FORMATION 1; LB, Luria–Bertani; LRR-RLK, leucine-rich repeat receptor-like kinase; OD, optical density; ORF, open reading frame; PCR, polymerase chain reaction; qRT-PCR, quantitative reverse transcription-PCR; SDI, shoot derived inhibitor; SUNN, SUPER NUMERIC NODULES; sym29, SYMBIOSIS29; WT, wild-type; YMB, Yeast Mannitol Broth |
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