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本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看

玉砌的強(qiáng)

金蟲 (正式寫手)

應(yīng)助: 5 (幼兒園)
金幣: 227.7
散金: 10
紅花: 2
帖子: 408
在線: 98.7小時(shí)
蟲號: 1909252
注冊: 2012-07-25
性別: GG
專業(yè): 有機(jī)金屬化學(xué)

[求助] 求大神幫忙翻譯三段話

Seed Germination and Seedling Establishment Assays
After surface sterilization of the seeds, stratification was conducted in the dark
at 4°C for 3 d. Next, ;100 seeds of each genotype were sown on MS plates
lacking or supplemented with 0.5 mM ABA, 150 mM NaCl, or 400 mM
mannitol. In the analyses of iDDA1 lines, 1 mMABAwas used and b-estradiol
was added to medium at 10 mM final concentration as stated. To score seed
germination, radicle emergence was analyzed at 72 and 96 h after sowing.
Seedling establishment was scored after 5 d as the percentage of seeds that
developed green expanded cotyledons and the first pair of true leaves.
Root Growth Assays
Seedlings were grown on vertically oriented MS plates for 4 to 5 d. Afterward,
20 plants were transferred to new MS plates lacking or supplemented
with 10 mM ABA. The plates were scanned on a flatbed scanner
after 10 d to produce image files suitable for quantitative analysis of root
growth using ImageJ version 1.37 software
Phylogenetic Analysis
DDA1 homologs were searched in different databases, Plaza (https://
bioinformatics.psb.ugent.be/plaza/), Phytozome (https://www.phytozome.net),
Gramene (https://www.gramene.org), TAIR (https://www.arabidopsis.org), and
GenBank (https://www.ncbi.nlm.nih.gov), using web-hosted BLAST applications
(https://blast.ncbi.nlm.nih.gov/Blast.cgi). All truncated DDA1 sequences
were excluded in subsequent analyses. Sequences were aligned using
ClustalW2 (https://www.ebi.ac.uk/Tools/msa/clustalw2/) with the Gonnet
protein comparison matrix and default parameters (gap opening penalty of
10 and gap extension penalty of 0.1 for initial pairwise alignment; gap
opening penalty of 10 and gap extension penalty of 0.2 for the multiple
alignment; with residue-specific and hydrophilic residue penalties, a gap
separation distance of 4 and a 30% delay divergent cutoff) and then
manually inspected to check for possible misalignments (Supplemental
Data Set 1). The best model fitting the alignment was selected using
ProtTest (Abascal et al., 2005), which corresponded to a Jones-Taylor-
Thornton model for amino acid substitution with a discrete gamma distribution
(four categories), a proportion of invariant, and an initial BIONJ tree.
This model was used to conduct a maximum likelihood analysis with
bootstrapping using 1000 replicates using the MEGA 5 (https://www.
megasoftware.net/) software. The gaps/missing treatment parameter was
set to complete deletion. The more distant homologs, from Selaginella
moellendorffii and Picea glauca, were used as an outgroup to root the
resulting phylogenetic tree.

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從心開始，從新開始......

1樓 2014-12-08 21:29:57

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

zawz99

新蟲 (初入文壇)

翻譯EPI: 1
應(yīng)助: 0 (幼兒園)
金幣: 14
帖子: 7
在線: 1.3小時(shí)
蟲號: 3584381
注冊: 2014-12-09

【答案】應(yīng)助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
玉砌的強(qiáng): 金幣+15, 翻譯EPI+1, ★★★很有幫助 2014-12-09 21:54:10

種子萌發(fā)和幼苗建立測定種子表面消毒后，分層在黑暗中進(jìn)行在4℃下3天。接著，100種子每種基因型的播種在MS平板缺乏或補(bǔ)充有0.5mM的ABA，150 mM氯化鈉，或400毫甘露醇。在iDDA1行分析，使用與1 mMABAwas B-雌二醇加入到培養(yǎng)基中，在10mM的最終濃度如所述。得分種子發(fā)芽，胚根出現(xiàn)在播種后72和96小時(shí)進(jìn)行分析。苗成立了進(jìn)球后5天作為種子的百分比綠色發(fā)展子葉擴(kuò)大和第一對真葉。根生長測定幼苗生長在垂直取向的MS平板進(jìn)行4至5天。隨后，20株植物被轉(zhuǎn)移至新的MS平板缺乏或補(bǔ)充用10mM的ABA。將平板上掃描平板掃描儀10 d后產(chǎn)生圖像文件適用于根的定量分析使用ImageJ1.37版本軟件的增長系統(tǒng)發(fā)育分析DDA1同源進(jìn)行檢索在不同的數(shù)據(jù)庫，廣場上（http：//bioinformatics.psb.ugent.be/plaza/），Phytozome（https://www.phytozome.net）Gramene（https://www.gramene.org），TAIR（https://www.arabidopsis.org），和GenBank中的（https://www.ncbi.nlm.nih.gov），使用Web托管應(yīng)用BLAST（https://blast.ncbi.nlm.nih.gov/Blast.cgi）。所有截?cái)郉DA1序列被排除在隨后的分析。序列使用對齊ClustalW2（https://www.ebi.ac.uk/Tools/msa/clustalw2/）與貢內(nèi)特蛋白質(zhì)比較矩陣和默認(rèn)參數(shù)（空位開放罰0.1初始配對比對10和缺口延伸罰分;差距所述多個(gè)開口的0.210和缺口延伸罰點(diǎn)球排列;與殘基的具體和親水殘基的處罰，間隙4分離距離和30％的延遲發(fā)散截止），然后手動檢查，以檢查可能的偏差（補(bǔ)充數(shù)據(jù)集1）。使用被選為最佳模型擬合的對齊ProtTest（阿瓦斯卡爾等人，2005），其對應(yīng)于一個(gè)瓊斯Taylor-氨基酸替代桑頓模型離散伽瑪分布（4種），的不變的比例，和一個(gè)初始BIONJ樹。這種模型被用來進(jìn)行最大似然分析引導(dǎo)使用1000次重復(fù)使用的MEGA5（HTTP：// WWW。megasoftware.net/）軟件。差距/缺少治療參數(shù)為設(shè)置完成刪除。更遙遠(yuǎn)的同源物，從卷柏moellendorffii和云杉青岡，被用作外群根的導(dǎo)致進(jìn)化樹。

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2樓2014-12-09 12:58:21