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轉(zhuǎn)身丶忘不掉銅蟲 (小有名氣)
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3.7. Cell morphology adhered on PLGA/PHBV scaffold As shown in Fig. 7, the cell morphology of hMSCs grown on three kinds of scaffolds showed some differences at 12 h. The cells had grown into the surface by forming filopodia between some apertures. Most of the cells seemed to prefer to adhere onto the surface of PHBV microspheres than onto the morphology of PLGA matrix made by NaCl particles. This point can be approved by graphs C, D, E, and F in Fig. 7. Most of the visible cells were lain on or between PHBV microspheres. 5. Conclusion In this study, we set up a new model which was composed of PLGA and PHBV for bone repair. After incorporating PHBV microspheres into PLGA scaffold, morphology, pore size distribution,porosity, compressive strength and in vitro experiments were employed to evaluate it. The PLGA/PHBV scaffold has good pore interconnectivity and macropores which is suitable for cells to grow in. Besides, by mixing the PHBV microspheres into PLGA matrix, the compressive strength of the scaffold was improved obviously while the morphology was not destroyed. The in vitro evaluation showed that the new model can facilitate the cells to proliferate well. In a word, the PLGA/PHBV model is worth further studying for a bone repair application. |
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3.7.黏附于PLGA/PHBV支架材料的細(xì)胞形貌 如圖7所示，在三種支架材料上生長(zhǎng)的hMSCs細(xì)胞形貌在12小時(shí)時(shí)顯示出某些不同。hMSCs細(xì)胞通過(guò)在某些孔隙間形成絲狀偽足而生長(zhǎng)進(jìn)入表面。大多數(shù)的細(xì)胞看起來(lái)更傾向于黏附在PHBV微球的表面而不是由NaCl粒子形成的PLGA集體的表面。圖7中C、D、 E及F圖可以證明這一點(diǎn)。大多數(shù)可見的hMSCs細(xì)胞位于PHBV微球上，或者在PHBV微球之間。 5. 結(jié)論 在本研究中，我們建立起一個(gè)由PLGA和PHBV構(gòu)筑的新模型從而實(shí)現(xiàn)骨修復(fù)。當(dāng)PHBV微球被引入PLGA支架材料后，我們通過(guò)形貌、孔徑分布、孔隙率、壓縮強(qiáng)度和體外實(shí)驗(yàn)等參數(shù)來(lái)評(píng)價(jià)這一新模型。PLGA/PHBV支架材料具有很好的孔隙連接性，同時(shí)富含適合細(xì)胞生長(zhǎng)的大孔。此外，通過(guò)將PHBV微球引入PLGA基體中，支架材料的壓縮強(qiáng)度顯著提升，而形貌則保持不變。體外評(píng)價(jià)顯示這一新模型可以很好地促進(jìn)細(xì)胞增殖�？偠灾�，PLGA/PHBV模型作為骨修復(fù)材料值得進(jìn)一步研究。 |
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