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[求助]
一篇JACS文章摘要求翻譯
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本人基本看明白了,但是書面翻譯的時(shí)候總是說不成流利的中文,求大神幫翻譯成漂亮的中文,金幣可以追加! 題目Finding the Switch: Turning a Baeyer–Villiger Monooxygenase into a NADPH Oxidase By a targeted enzyme engineering approach, we were able to create an efficient NADPH oxidase from a monooxygenase. Intriguingly, replacement of only one specific single amino acid was sufficient for such a monooxygenase-to-oxidase switch—a complete transition in enzyme activity. Pre-steady-state kinetic analysis and elucidation of the crystal structure of the C65D PAMO mutant revealed that the mutation introduces small changes near the flavin cofactor, resulting in a rapid decay of the peroxyflavin intermediate. The engineered biocatalyst was shown to be a thermostable, solvent tolerant, and effective cofactor-regenerating biocatalyst. Therefore, it represents a valuable new biocatalytic tool. |
木蟲 (正式寫手)
JIN CHONG

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