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cwq921

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[求助] 求職翻譯摘要，快畢不了業(yè)了

摘要：探討病毒Ad35-SF感染T細(xì)胞后表達(dá)SIRPα-Fc（SF）融合基因蛋白對(duì)T細(xì)胞功能的影響。以包裝含有SF基因的腺病毒Ad35-SF感染Jurkat細(xì)胞48h，Western blotting檢測(cè)結(jié)果顯示SF蛋白大小約為48kD,與理論值吻合，ELISA檢測(cè)表明該蛋白在細(xì)胞上清中的表達(dá)量為19.1μg/ml。Ad35-SF（MOI=1）感染Jurkat細(xì)胞8h后，CD47陽(yáng)性比例從97.06％下降到15.32％；當(dāng)Ad35-SF感染復(fù)數(shù)增加至5,10時(shí)，CD47陽(yáng)性比率進(jìn)一步降低至2.94%和1.73%。相對(duì)參照組，Ad35-SF感染Jurkat細(xì)胞48h后其增殖效果提高了29％，對(duì)肝癌細(xì)胞株Hep 3B的殺傷作用提升25.6％。該研究表明構(gòu)建的病毒Ad35-SF能有效感染Jurkat細(xì)胞并分泌SF蛋白，從而封閉其表面的CD47。感染后的Jurkat細(xì)胞會(huì)繼續(xù)分泌SF蛋白并封閉其它未被感染的Jurkat細(xì)胞，從而顯著提高Jurkat細(xì)胞的增殖能力和對(duì)肝癌細(xì)胞的殺傷作用。

高手們幫幫忙，確實(shí)英語(yǔ)差，馬上要畢業(yè)了，還沒(méi)投稿，快幫幫忙吧，謝謝

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1樓 2014-12-15 10:37:39

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【答案】應(yīng)助回帖

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cwq921: 金幣+50, ★★★★★最佳答案, 幫實(shí)驗(yàn)室的，謝謝啦！ 2014-12-15 12:45:43

Abstract: To investigate the effect of expression of SIRPα-Fc (SF), a fusion protein encoded by adenovirus Ad35-SF, on T cell function, Jurkat cells were infected with adenovirus Ad35-SF for 48 h. Western blotting analysis showed that the expressed fusion protein SF was about 48 kD, which was consistent with the calculated value. ELISA assay showed that the concentration of the fusion protein in the culture medium was 19.1 μg/ml. CD47-positive cells decreased dramatically from 97.06% to 15.32% when Jurkat cells were infected with Ad35-SF [multiplicity of infection (MOI)=1] for 8h, and further decreased to 2.94% and 1.73%, respectively, if MOI was increased to 5 and 10. The proliferation of Jurkat cells 48 h after Ad35-SF infection increased by 29% and increased the killing effect of of the infected Jrukat cells to hepatocellular carcinoma cell line Hep3B by 25.6%, as compared with the controls. The results of this study showed that the adenovirus Ad35-SF we made can efficiently infect Jurkat cells and express functional secretory protein SF, thereby blocking the cell surface marker CD47. In addition, the infected Jurkat cells will continue to secrete SF protein and block other uninfected Jurkat cells, which can significantly improve the proliferation of Jurkat cells and their cytotoxicity to hepatocellular carcinoma cells.

注：
1、沒(méi)有完全按照你的中文一對(duì)一翻譯（盡可能按你的原話），而是根據(jù)英文表達(dá)習(xí)慣按照你要表達(dá)的內(nèi)容做了適當(dāng)調(diào)整。
2、你的中文有許多地方需要調(diào)整，如第一句和第二句之間有一種目的關(guān)系，故而不宜用句號(hào)。倒是后面一連串的逗號(hào)需要重新斷句。再如”細(xì)胞上清中的表達(dá)量為19.1μg/ml“，我明白你在說(shuō)什么，但嚴(yán)格說(shuō)來(lái)所謂細(xì)胞上清應(yīng)該是指離心后的培養(yǎng)基（相對(duì)于條件培養(yǎng)基conditional medium），故而我在翻譯時(shí)用了culture medium而非cell supernatants；另外，你確定蛋白濃度是19.1 μg/ml而不是19.1 ng/ml嗎？

希望有幫助，盡早畢業(yè)！

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2樓2014-12-15 12:09:56

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