永峰1230

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[求助] 序列提交 reading frame shifts 已有1人參與

序列提交后收到郵件：
We have noticed that some or all of the sequences contain internal stop
codons and/or reading frame shifts (insertions/deletions based on BLAST
similarity search results and/or an alignment).

This generally indicates problems such as:

  - errors in the nucleotide sequence
  - sequences have not been trimmed of low quality
  - problems with feature annotation which may be due to:
translation in the wrong reading frame or on the wrong strand
translation does not have the correct exon spans
translation includes noncoding sequence, such as introns or UTR's

If the sequences and/or annotation are incorrect, please resubmit
with the corrected files. If the information is correct, please explain
the presence of the internal stop codons and/or frame shifts.

If you are unable to determine the correct sequence and/or annotation
for your sequence(s), they can still be processed and the following
comment will be added to your records:

COMMENT  GenBank staff is unable to verify sequence and/or annotation
      provided by the submitter.

In addition, the record(s) will indicate that the data are unverified and
your sequence(s) will not be included in NCBI BLAST databases.

Please either:

[1] resubmit with correct sequence(s) and/or annotation
or
[2] confirm that your sequence(s) should be processed as unverified.
試過(guò)好幾種方法，，都是這種郵件，，，該怎么弄呢？？？

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1樓 2014-12-19 21:04:19

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wizardfan

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感謝參與，應(yīng)助指數(shù) +1
永峰1230: 金幣+6, ★★★很有幫助 2014-12-30 10:09:16

"試過(guò)好幾種方法", what have you tried?

It says clear in the email:
1. check why there are stop codons in your sequence

2. or just to say it is unverified sequence

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» 本帖已獲得的紅花（最新10朵）

永峰1230

2樓2014-12-20 10:20:26

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永峰1230

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2樓: Originally posted by wizardfan at 2014-12-20 10:20:26
"試過(guò)好幾種方法", what have you tried?

It says clear in the email:
1. check why there are stop codons in your sequence

2. or just to say it is unverified sequence

or just to say it is unverified sequence ，，是什么意思呢？是指沒(méi)有人表達(dá)的嗎？還是說(shuō) 這只是我擴(kuò)增測(cè)序出來(lái)的，，沒(méi)有人進(jìn)一步證實(shí)的？

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3樓2014-12-30 10:09:07

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wizardfan

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永峰1230: 金幣+6, ★★★很有幫助 2014-12-31 11:02:54

unverified = 沒(méi)進(jìn)一步證實(shí), it can be verified by you or someone else. But as it is a new sequence, you are the only person to verify it.
If I see an unverified sequence in NCBI, I won't use it as the quality of the sequence is unknown.

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4樓2014-12-31 10:43:16

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永峰1230

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引用回帖:

4樓: Originally posted by wizardfan at 2014-12-31 10:43:16
unverified = 沒(méi)進(jìn)一步證實(shí), it can be verified by you or someone else. But as it is a new sequence, you are the only person to verify it.
If I see an unverified sequence in NCBI, I won't use it as th ...

如果回復(fù)郵件說(shuō)是 unverified，能夠提交上去嗎？還需要進(jìn)一步做什么嗎？

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5樓2014-12-31 11:02:46

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wizardfan

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永峰1230: 金幣+8, ★★★很有幫助, 謝謝你，，thank you 2015-01-03 11:56:18

according to the content of NCBI email, it should be fine to submit your sequence while confirming they are unverified. But there is no point for doing so. It is up to you. The only thing you will get is a NCBI accession for your sequence, that is it, probably no one will use it.

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6樓2014-12-31 20:52:16

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mary651

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樓主問(wèn)題解決了嗎，我也遇到同樣的問(wèn)題，求指教

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7樓2015-01-23 23:26:49

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永峰1230

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7樓: Originally posted by mary651 at 2015-01-23 23:26:49
樓主問(wèn)題解決了嗎，我也遇到同樣的問(wèn)題，求指教

還沒(méi)有了，回復(fù)郵件還是那樣子，這幾天忘記弄了。。

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8樓2015-01-24 08:43:27

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車彥辰

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8樓: Originally posted by 永峰1230 at 2015-01-24 08:43:27
還沒(méi)有了，回復(fù)郵件還是那樣子，這幾天忘記弄了。。...

求助啊，我的也是這樣啊。你的解決了么？？？

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9樓2015-04-03 16:30:53

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yxx20060429

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7樓: Originally posted by mary651 at 2015-01-23 23:26:49
樓主問(wèn)題解決了嗎，我也遇到同樣的問(wèn)題，求指教

您好！我也遇到了Internal stop codons have been detected in the conceptual translation
of the following coding regions這個(gè)問(wèn)題，請(qǐng)問(wèn)您是怎么解決的，畢業(yè)在即，文章等著序列釋放才接受。望慷慨相助。yxx20060429@163.com

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10樓2018-04-11 21:39:03

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