| 4 | 1/1 | 返回列表 |
| 查看: 1058 | 回復(fù): 3 | |||
橐橐22金蟲 (小有名氣)
|
[求助]
FITC標(biāo)記伯胺時(shí)所用緩沖液的問題 已有1人參與
|
| 我現(xiàn)在要用FITC(異硫氰酸熒光素)衍生胺類樣品,但是文獻(xiàn)中衍生時(shí)所用的緩沖有磷酸鹽、碳酸鹽、硼酸鹽和Tris-HCl,雖然我知道衍生反應(yīng)在堿性條件下是最好的,但是還是想問這幾種緩沖在FITC熒光衍生反應(yīng)中有什么不同嗎,用哪一種最恰當(dāng)呢? |

新蟲 (初入文壇)
|
我用過DMSO做溶劑溶解FITC的溶劑,可以成功標(biāo)記上伯胺 方法很簡(jiǎn)單: 下面是我的文章內(nèi)容相關(guān)部分,可作參考 A fluorescence agent, FITC, was used to identify the presence of the amine functional group on the AMNPs surface. First, 5 mg of AMNPs were added into 1.5 mL EP tube and washed three times with 1 mL of DMSO. 1 mL of FITC solution (1 mg/mL, dissolved in DMSO) was added, and the mixture was sonicated for suspension for 2 h in a dark environment at room temperature. |

|
本帖內(nèi)容被屏蔽 |

| 4 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研]
|
2117205181 2026-03-21 | 8/400 |
|
|---|---|---|---|---|
|
[考研] 293求調(diào)劑 +3 | 濤濤Wjt 2026-03-22 | 5/250 |
|
|
[考研] 求調(diào)劑 +6 | 十三加油 2026-03-21 | 6/300 |
|
|
[考研] 308求調(diào)劑 +3 | 墨墨漠 2026-03-21 | 3/150 |
|
|
[考研] 070300化學(xué)求調(diào)劑 +3 | 苑豆豆 2026-03-20 | 3/150 |
|
|
[考研] 一志愿華中科技大學(xué)071000,求調(diào)劑 +4 | 沿岸有貝殼6 2026-03-21 | 4/200 |
|
|
[考研] 求調(diào)劑 +3 | 13341 2026-03-20 | 3/150 |
|
|
[考研] 求助 +5 | 夢(mèng)里的無言 2026-03-21 | 6/300 |
|
|
[考研] 336求調(diào)劑 +5 | rmc8866 2026-03-21 | 5/250 |
|
|
[考研] 22 350 本科985求調(diào)劑,求老登收留 +3 | 李軼男003 2026-03-20 | 3/150 |
|
|
[考研] 機(jī)械專碩299求調(diào)劑至材料 +3 | kkcoco25 2026-03-16 | 4/200 |
|
|
[考研] 265求調(diào)劑 +3 | Jack?k?y 2026-03-17 | 3/150 |
|
|
[考研] 332求調(diào)劑 +4 | ydfyh 2026-03-17 | 4/200 |
|
|
[考研]
|
.6lL 2026-03-18 | 8/400 |
|
|
[考研] 317求調(diào)劑 +5 | 申子申申 2026-03-19 | 9/450 |
|
|
[考研] 工科材料085601 279求調(diào)劑 +7 | 困于星晨 2026-03-17 | 9/450 |
|
|
[考研] 招收調(diào)劑碩士 +4 | lidianxing 2026-03-19 | 12/600 |
|
|
[考研] 0703化學(xué)調(diào)劑 +4 | 18889395102 2026-03-18 | 4/200 |
|
|
[考研] 材料工程專碩調(diào)劑 +5 | 204818@lcx 2026-03-17 | 6/300 |
|
|
[考研] 085601專碩,總分342求調(diào)劑,地區(qū)不限 +5 | share_joy 2026-03-16 | 5/250 |
|