 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 1883 | 回復(fù): 4 | |||
| 當(dāng)前只顯示滿足指定條件的回帖，點擊這里查看本話題的所有回帖 | |||
北海艦隊鐵蟲 (初入文壇)
|
[交流]
血淚求助：Trinity的normalize 已有2人參與
|
||
|
鄙人最近用Trinity軟件normalize一個trim后的數(shù)據(jù)，奈何一直報錯如下： Error, pairs.K25.stats is empty. Be sure to check your fastq reads and ensure that the read names are identical except for the /1 or /2 designation. at /diag/home/aubzyy/trinityrnaseq_r20140717/util/insilico_read_normalization.pl line 865. 這是咋回事啊，俺是新手，跪求大俠出手相助！ 我的數(shù)據(jù)是Pair-end。。。   |
回復(fù)此樓» 猜你喜歡
不合理蛙科研實驗中的趣事：實驗器材的 “烏龍”
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗中的趣事：和實驗材料的 “斗智斗勇”
已經(jīng)有0人回復(fù)
-
化學(xué)工程及工業(yè)化學(xué)論文潤色/翻譯怎么收費?
已經(jīng)有89人回復(fù)
-
蛋白質(zhì)檢測：精準(zhǔn)分析，解鎖生物分子的密碼
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗之小鼠實驗：嚴(yán)謹(jǐn)設(shè)計，解析生命機制的重要載體
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗之重金屬檢測：精準(zhǔn)篩查，守護(hù)健康與環(huán)境的防線
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗之“蛙測重金屬我背鍋三千”
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗之“鼠逃三次我發(fā)三篇SCI”
已經(jīng)有0人回復(fù)
-
納米粒度分析
已經(jīng)有3人回復(fù)
-
林科院林化所招收材料與化工專業(yè)碩士，坐標(biāo)南京
已經(jīng)有0人回復(fù)
wizardfan
至尊木蟲 (著名寫手)
- MolEPI: 4
- 應(yīng)助: 599 (博士)
- 貴賓: 1.818
- 金幣: 24632.2
- 散金: 197
- 紅花: 48
- 沙發(fā): 2
- 帖子: 2254
- 在線: 400.7小時
- 蟲號: 1879241
- 注冊: 2012-07-05
- 性別: GG
- 專業(yè): 生物信息學(xué)
★
小木蟲: 金幣+0.5, 給個紅包，謝謝回帖
小木蟲: 金幣+0.5, 給個紅包，謝謝回帖
|
it says that the name of sequences in your fastq can not match In fastq format, Line 1 begins with a '@' character and is followed by a sequence identifier and an optional description (like a FASTA title line). e.g. @HWI-EAS209_0006_FC706VJ:5:58:5894:21141#ATCACG/1 another entry would be something like @HWI-EAS209_0006_FC706VJ:5:58:5894:21141#ATCACG/2 |
3樓2015-01-16 08:09:27
2樓2015-01-15 10:29:15
北海艦隊
鐵蟲 (初入文壇)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 46.3
- 帖子: 26
- 在線: 17.3小時
- 蟲號: 1634534
- 注冊: 2012-02-22
- 專業(yè): 病毒學(xué)
|
Thank you. But my sequence starts like this: @SRR1559285.21 HWI-ST531R:238:C346VACXX:5:1101:1193:2038 length=101 CCTTCATTCATGTGCTCACGCCAGTATGCAGCATTTTTAACAGCACAGTCCCAGT +SRR1559285.21 HWI-ST531R:238:C346VACXX:5:1101:1193:2038 length=101 @CCFFFFFGHHGHIIJJJ4FHIJJGIIJJIIJIJJJJJHIJJJIIJJJHHJJJJI @SRR1559285.22 HWI-ST531R:238:C346VACXX:5:1101:1126:2077 length=101 CCTATTCATTTACAGCAGAAGTGCTATTTCTTATTCATGATGTACAAGTCGTTGTTCCGTGAAAATGAAACAGAGACGGG CGTCGTAGGATCCTTAGACGG +SRR1559285.22 HWI-ST531R:238:C346VACXX:5:1101:1126:2077 length=101 CCCFFFFFHHGHHFIJJJHIIIHIJJJIJIJJIHJGGIFHIIGGIIJJFGHGEGHGIGGDGGGJJIIJGHIIIHJJHHHF FD?BDBBBDCCCDDDDC:@@B @SRR1559285.23 HWI-ST531R:238:C346VACXX:5:1101:1228:2083 length=101 CAGACGAATTATAGAAAGGCTGTTTAATTAGAAATTTACTTAATCCAAGTTTTGGCTGAAATGGATTGAAGTTTGTCCTG GAATGTTTCGAACACATCTGT +SRR1559285.23 HWI-ST531R:238:C346VACXX:5:1101:1228:2083 length=101 CCCFFFFFHFHGFIBHIJIJJJJGIGAHIEIHIHHIHIFIIIIIJJJEHHGIJIIHIIGJGIII@FHJGIGCAHEEHHFF FFFCEEEECDDDBDDDDCD:@ @SRR1559285.24 HWI-ST531R:238:C346VACXX:5:1101:1246:2099 length=101 TGAAACTGAGGAATGTATGCCTCTCACGGTGACCCTGTCTCACCGTCTGAACCAAAAAATTGCTGAGCTTCGTAGAGATG GTACATTCTGGTGGGCAAGAC +SRR1559285.24 HWI-ST531R:238:C346VACXX:5:1101:1246:2099 length=101 @@@DF;DDBFFFFGGGHIJJAHGHIGEHGACGIJFIGIIGGIJJJHGIJIIJJEIIIIGGCACEEEFDEFFFDEC;@CCC D@DCDFDDEEDCDDDB?@B<9 @SRR1559285.25 HWI-ST531R:238:C346VACXX:5:1101:1179:2100 length=101 Is this okay? I am a first year graduate, thank you for your patience. |
4樓2015-01-19 13:40:24
wizardfan
至尊木蟲 (著名寫手)
- MolEPI: 4
- 應(yīng)助: 599 (博士)
- 貴賓: 1.818
- 金幣: 24632.2
- 散金: 197
- 紅花: 48
- 沙發(fā): 2
- 帖子: 2254
- 在線: 400.7小時
- 蟲號: 1879241
- 注冊: 2012-07-05
- 性別: GG
- 專業(yè): 生物信息學(xué)
★
小木蟲: 金幣+0.5, 給個紅包，謝謝回帖
小木蟲: 金幣+0.5, 給個紅包，謝謝回帖
|
how sure you are that this is a pair-end data? Normally two files for pair-end data, named like ***_1.fastaq and ***_2.fastq. In ***_1.fastq, the headers of sequences are like ********/1, so as in the second file. There is a public server for you to use Trinity https://galaxy.cbio.mskcc.org/ |
5樓2015-01-20 10:18:19
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 一志愿吉林大學(xué)材料與化工303分求調(diào)劑 +3 | 為學(xué)666 2026-03-24 | 3/150 |
|
|---|---|---|---|---|
|
[考研] 生物學(xué)學(xué)碩求調(diào)劑 +7 | 小羊睡著了? 2026-03-23 | 10/500 |
|
|
|
[考研] 一志愿武理085500機械專業(yè)總分300求調(diào)劑 +3 | an10101 2026-03-24 | 7/350 |
|
|
|
[考研] 收08調(diào)劑生 +6 | komorebi69 2026-03-18 | 6/300 |
|
|
|
[考研] 080500求調(diào)劑 +3 | zzzzfan 2026-03-24 | 3/150 |
|
|
|
[考博] 申博26年 +4 | 八6八68 2026-03-19 | 4/200 |
|
|
|
[考研] 材料與化工328分調(diào)劑 +4 | 。，。，。，。i 2026-03-23 | 4/200 |
|
|
|
[考研] 344求調(diào)劑 +3 | desto 2026-03-24 | 3/150 |
|
|
|
[考研] 接收2026碩士調(diào)劑(學(xué)碩+專碩) +4 | allen-yin 2026-03-23 | 6/300 |
|
|
|
[考研] 333求調(diào)劑 +6 | 87639 2026-03-21 | 10/500 |
|
|
|
[考研] 291求調(diào)劑 +5 | 孅華 2026-03-22 | 5/250 |
|
|
|
[考研] 尋找調(diào)劑 +4 | 倔強芒? 2026-03-21 | 4/200 |
|
|
|
[考研] 319求調(diào)劑 +4 | 小力氣珂珂 2026-03-20 | 4/200 |
|
|
|
[考研] 311求調(diào)劑 +3 | 勇敢的小吳 2026-03-20 | 3/150 |
|
|
|
[考研] 一志愿重慶大學(xué)085700資源與環(huán)境總分308求調(diào)劑 +7 | 墨墨漠 2026-03-20 | 7/350 |
|
|
|
[考研] 0805材料320求調(diào)劑 +3 | 深海物語 2026-03-20 | 3/150 |
|
|
|
[考研] 一志愿 西北大學(xué) ，070300化學(xué)學(xué)碩，總分287，雙非一本，求調(diào)劑。 +3 | 晨昏線與星海 2026-03-18 | 3/150 |
|
|
|
[考研] 22408 344分 求調(diào)劑 一志愿 華電計算機技術(shù) +4 | solanXXX 2026-03-20 | 4/200 |
|
|
|
[考研] 317求調(diào)劑 +5 | 申子申申 2026-03-19 | 9/450 |
|
|
|
[考研]
 材料與化工 322求調(diào)劑
+4
|
然11 2026-03-19 | 4/200 |
|