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stevenlee8356銀蟲 (正式寫手)
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[求助]
NCBI提交基因組序列,工作人員回信,大家看看是什么意思,謝謝啦。。。 已有1人參與
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前段時(shí)間給NCBI提交了一段全基因組序列,過了幾天工作人員給回信過來,大家看看下面的回信是什么意思,謝謝啦。。! Dear XXXXX, Thank you for your Lactobacillus plantrum NMR-5 genome submission. Before we can assign your accession number, there are a few issues that require your attention: [1] This appears to be a complete and circularized genome. Please let us know if this is not the case. [2] If you do not plan to submit your own annotation, we would like to run your complete unannotated genome and plasmid through the NCBI prokaryotic annotation pipeline. Having annotation on the complete bacterial genomes is very useful to the scientific community. [3] If you would like us to annotate your genome, please let us know if you would like to review the data or for us to release the annotation without your review. [4] Have you registered a genome project and BioSample for your genomes? If you have, please provide the ID numbers that were returned. If you have not, please register your genome and locus_tag prefix at: https://submit.ncbi.nlm.nih.gov/subs/bioproject/ Register your BioSample at: https://submit.ncbi.nlm.nih.gov/subs/biosample/ [5] Please provide the following information: --coverage (if appropriate) --Was this assembly based on a reference genome? If so, please provide the accession number for the reference genome that was used. [6] It has been brought to our attention that it can be extremely difficult to find the source DNA/bacteria for many of the bacteria for which there are complete genomes. Sometimes the submitting centers are not the ones who have the source DNA or bacteria strain. In light of this difficulty, we are now asking submitters to provide DNA/bacteria source information to us. A single line is satisfactory-- 'Source DNA is available from....'; 'Bacteria available from...', where a name and email address or culture collection information is given. Please let us know if you have any questions about these comments. Sincerely, Leigh ******************************************************************* Leigh A. Riley, Ph.D. The GenBank Direct Submission Staff Bethesda, Maryland USA !!!Please send all .sqn files to us using: http://www.ncbi.nlm.nih.gov/proj ... t/genome_submit.cgi !!! genomes@ncbi.nlm.nih.gov (for updates/replies to GenBank entries) info@ncbi.nlm.nih.gov (for general questions regarding GenBank) ******************************************************************* |
金蟲 (著名寫手)
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【1】你提交的可能是環(huán)狀的基因組序列,如果不是,你要及時(shí)通知我們 【2】如果你不打算注釋你提交的序列,我們將幫你注釋,注釋后的基因組信息是一個(gè)非常有用的科研工具。 【3】如果你讓我們來注釋基因組信息,請(qǐng)告知我們你是否要核查注釋后的數(shù)據(jù)信息或者我們注釋后就直接將信息披露出去 【4】你以前為你的基因組提交過相應(yīng)的生物樣品嗎?如果有,請(qǐng)告知我們當(dāng)時(shí)我們返給你的ID號(hào)。注:你的基因組是新的,那么所對(duì)應(yīng)的生物樣品也該是未曾在NCBI上登記的,所以要登記下。 【5】請(qǐng)?zhí)峁┫嚓P(guān)信息:1.完整度2你提供的序列信息是否由相關(guān)序列組裝起來的,如果是,請(qǐng)?zhí)峤荒切┬蛄械牡卿浱?hào) 總之,你要讓別人相信你提交的數(shù)據(jù),你要提供更詳細(xì)的信息,對(duì)其進(jìn)行注釋或由他們注釋,其次你要提供該基因組信息的DNA來源,如哪一種菌株等!!GOOD LUCK! |

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銀蟲 (正式寫手)
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新蟲 (初入文壇)
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