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本帖產生 1 個翻譯EPI ，點擊這里進行查看

huojinlong8610

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PPARD基因在動物脂肪代謝過程中起重要作用，本研究以豬為實驗動物，通過RT-PCR方法擴增得到PPARD基因全長編碼區(qū)序列，在編碼區(qū)第95位檢測到G/A兩種核苷酸，序列提交GenBank數據庫獲得登錄號“KP757025和KP757026”。生物信息學分析顯示：該基因編碼462個氨基酸，蛋白分子量為49.74 kD，等電點為7.53。蛋白質結構分析PPARD存在2個保守域，無跨膜結構，不存在信號肽，存在1個亮氨酸富集的核輸出信號，其N端、C端均親水；亞細胞定位顯示該蛋白分泌到細胞周質的概率為69.6%。系統(tǒng)進化分析表明，豬與牛、家犬的親緣關系較近。利用qPCR檢測了PPARD基因mRNA在豬14個組織中的表達量，發(fā)現在肝臟、耳朵、脾臟、小腸中表達量較高。本研究為進一步研究豬PPARD基因的表達調控奠定研究基礎。
PPARD as the main fat metabolism genes, for studying the regulation and function of PPARD in pigs, PPARD cDNA including the coding sequence was obtained by RT-PCR with the RNA isolated from pigs and submitted to GenBank with the access number. Bioinformatics analysis showed that the PPARD gene encode a protein of 462 amino acids with a predicted molecular weight of 49.74 kD and the isoelectric point of 7.53. Protein structure analysis showed that PPARD protein contains two conserved domain, no transmembrane regions and signal peptide and had a leucine-rich nuclear export signals． Hydrophobicity analysis indicate that the N-terminus and C-terminus are hydrophilic. The probability of secreting into periplasmic is 69.6%. Phylogenetic results indicated that the pig has the closest relationship with cattle and dog. We detected G and A two kinds nucleotides at 95 nucleotide in the coding region of the PPARD gene and three genotypes were found: AA, AB, BB. We tested the PPARD mRNA expression in 14 tissues by qPCR, the result showed that the expression is high in liver, spleen, ear, small intestine. This study provides experimental basis for further study on the expression and regulation of PPARD gene.

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huojinlong8610: 金幣+100, 翻譯EPI+1 2015-02-11 15:28:27

PPARδ is a nuclear hormone receptor that governs a variety of biological processes, including fatty acid metabolism. For studying the regulation and function of PPARD in pigs, PPARδ cDNA including the coding sequence was obtained by RT-PCR with the RNA isolated from pigs and submitted to GenBank with the access number. Bioinformatics analysis showed that the PPARδ gene encode a protein of 462 amino acids with a predicted molecular weight of 49.74 kD and the isoelectric point of 7.53. Protein structure analysis showed that PPARδ protein contains two conserved domains and a leucine-rich nuclear export signal without transmembrane regions and signal sequence． Hydrophobicity analysis indicate that the N-terminus and C-terminus are hydrophilic. The probability of secreting into periplasmic is 69.6%. Phylogenetic results indicated that the pig has the closest relationship with cattle and dog. Two different nucleotides (A or G) at 95 nucleotide in the coding region of the PPARδ gene and three genotypes were found: AA, AB, BB. PPARδ mRNA expression in 14 tissues was tested by qPCR and the result showed that the expression was high in liver, spleen, ear and small intestine. This study provides experimental basis for further study on the expression and regulation of PPARδ gene.

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2樓2015-02-11 03:07:34