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After reaction with NHS-PEG-MAL, the peak area at the binding energy of 286.4 eV increases (Figure 1c and Table 2), and a new peak at the binding energy of 532.7 eV appears (Figure 2c and Table 2) because the introduced PEG chains contain abundant C-O bonds. In the C1s spectrum, the peak area at the binding energy of 286.4 eV keeps increasing after the covalent binding of RGD-containing peptides because of the contribution of C-N and C-O bonds in the peptides (Figure 1d and Table 2). In the O1s spectrum, the peak areas at the binding energy of 531.3 eV increase significantly (Figure 2d and Table 2) because the grafted RGD-containing peptides contain abundant N-CdO bonds. Taken together, XPS results of different PHBV films indicate the successful binding of RGD-containing peptides to ammonia plasma-treated PHBV surfaces through the cross-linker of bifunctional PEG. |
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愛與雨下: 金幣+1 2015-04-07 20:28:34
愛與雨下: 金幣+1 2015-04-07 20:28:34
| 與NHS-PEG-MAL反應(yīng)后,結(jié)合能的峰面積286.4電動(dòng)汽車的增加(圖1 c和表2)和一個(gè)新高峰結(jié)合能532.7 eV出現(xiàn)(圖2 c和表2),因?yàn)榻榻B了掛鉤鏈包含豐富的切斷債券。在c1,結(jié)合能的峰面積286.4 eV增加RGD-containing多肽共價(jià)結(jié)合后因?yàn)橛玫呢暙I(xiàn)和切斷的債券肽(圖1 d和表2)。在o1譜,峰值區(qū)域的結(jié)合能531.3 eV顯著增加(圖2 d和表2),因?yàn)榻又GD-containing肽含有豐富N-CdO債券。綜上所述,XPS結(jié)果表明成功綁定不同PHBRGDcontaining肽對(duì)氨plasma-treated PHBV的表面通過交聯(lián)雙官能掛鉤 |
2樓2015-04-03 17:07:22
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