版塊導(dǎo)航: 正在加載中...

登錄注冊

應(yīng)《網(wǎng)絡(luò)安全法》要求，自2017年10月1日起，未進(jìn)行實(shí)名認(rèn)證將不得使用互聯(lián)網(wǎng)跟帖服務(wù)。為保障您的帳號能夠正常使用，請盡快對帳號進(jìn)行手機(jī)號驗(yàn)證，感謝您的理解與支持！

24小時熱門版塊排行榜

返回列表

本帖產(chǎn)生 1 個翻譯EPI ，點(diǎn)擊這里進(jìn)行查看

Malyhon

新蟲 (小有名氣)

應(yīng)助: 0 (幼兒園)
金幣: 26.5
帖子: 56
在線: 13.9小時
蟲號: 3398024
注冊: 2014-09-04

[求助] 幾句論文求大神翻譯成中文謝謝

2.8. Fibrinogen Adsorption Test. Fibrinogen adsorption was
evaluated using enzyme-linked immunosorbent assay (ELISA) based on
the antigen-antibody interaction.36 The fresh whole blood of a healthy
volunteer was centrifuged at 3000 rpm to obtain platelet-poor plasma.
After being immersed in PBS at 37 C for 3 h, modified and unmodified
PHBV films were incubated in plasma at 4 C overnight. Then, all films
were blocked with 1% BSA (Sigma-Aldrich) at 37 C for 1 h, followed by
reacting with horseradish peroxidase (HRP) conjugated goat antihuman
fibrinogen (Sigma-Aldrich) at 37 C for 1 h. Then, TMB (3,30,5,50-
tetramethylbenzidine, Sigma-Aldrich) substrate was applied to react
with HRP until the solution in the well turned blue. Finally, 2M H2SO4
was added to each well to stop the reaction. The final color of the
solution was yellow, which could be read at the 450 nm with a microplate
reader. Each type of sample underwent six repeated measurements.
2.9. Statistical Analysis. The results of the cell viability assay and
fibrinogen adsorption test were analyzed by ANOVA, and the differences
observed between the samples were considered to be statistically
significant for P < 0.05.

回復(fù)此樓

» 猜你喜歡

299求調(diào)劑已經(jīng)有6人回復(fù)
286分人工智能專業(yè)請求調(diào)劑愿意跨考！已經(jīng)有5人回復(fù)
279求調(diào)劑已經(jīng)有3人回復(fù)
336求調(diào)劑已經(jīng)有3人回復(fù)
286求調(diào)劑已經(jīng)有3人回復(fù)
一志愿華中科技大學(xué)071000，求調(diào)劑已經(jīng)有3人回復(fù)
279分求調(diào)劑一志愿211 已經(jīng)有14人回復(fù)
332求調(diào)劑已經(jīng)有3人回復(fù)
0856材料專碩353求調(diào)劑已經(jīng)有3人回復(fù)
材料學(xué)學(xué)碩080502 337求調(diào)劑-一志愿華中科技大學(xué) 已經(jīng)有5人回復(fù)

1樓 2015-04-13 16:19:29

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

jiangguofeng

金蟲 (著名寫手)

翻譯EPI: 248
應(yīng)助: 41 (小學(xué)生)
金幣: 25886.4
紅花: 25
帖子: 2100
在線: 567.1小時
蟲號: 72410
注冊: 2005-06-04
專業(yè): 合成藥物化學(xué)

【答案】應(yīng)助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★
Malyhon: 金幣+10, 翻譯EPI+1, ★★★★★最佳答案 2015-04-27 14:46:02

2.8. 纖維蛋白原吸附試驗(yàn)。根據(jù)抗原-抗體交互作用，使用酶聯(lián)免疫吸附測定(ELISA)對纖維蛋白原吸附進(jìn)行了評價。36將健康志愿者的新鮮全血以3000rpm（每分鐘轉(zhuǎn)數(shù)）甩濾得到乏血小板血漿。于37℃的PBS中浸漬３小時之后,修飾和未修飾的PHBV膜于4℃血漿中培育過夜。然后,所有膜用1% BSA (西格瑪-奧德里奇產(chǎn)品）于37℃中封閉1小時,隨后37℃與辣根過氧化酶(HRP)結(jié)合的羊抗人纖維蛋白原(（西格瑪-奧德里奇產(chǎn)品）反應(yīng)1小時。然后TMB(3,30,5,50 -四甲基聯(lián)苯胺,西格瑪-奧德里奇產(chǎn)品）培養(yǎng)基用于與HRP反應(yīng)直到容器中的溶液變成藍(lán)色為止。最后,將2M的H2SO4添加到每個容器中以終止反應(yīng)。溶液最終的顏色為黃色,該數(shù)值可以用酶標(biāo)儀于450 nm讀取。每種樣品進(jìn)行六次反復(fù)測量。2.9.統(tǒng)計分析。細(xì)胞活力檢測和纖維蛋白原吸附試驗(yàn)通過統(tǒng)計學(xué)方差分析進(jìn)行的，樣品之間觀測到的差異P<0.05被認(rèn)為在統(tǒng)計上有顯著意義。

贊一下(1人)

回復(fù)此樓

2樓2015-04-15 10:04:21

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

相關(guān)版塊跳轉(zhuǎn) 我要訂閱樓主 Malyhon 的主題更新