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Malyhon新蟲 (小有名氣)
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[求助]
幾句論文求大神翻譯成中文 謝謝
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2.7. Serum Adsorption Tests by Optical Waveguide Lightmode Spectroscopy (OWLS). Nonspecific protein adsorption from serum was tested on unmodified and various modified PHBV films by OWLS.30,33 In brief, 2 wt % of PHBV was spin-coated onto waveguide chips, and subsequent surface modifications were carried out as described in Section 2.1. For comparison with the properties of MAL-PEG-grafted PHBV films, CH3OPEG-grafted PHBV films (PHBV-PEGOCH3) were prepared by the reaction of NH3-plasma-treated PHBV with 2 mM N-hydroxysuccinimidyl ester of methoxypoly-(ethylene glycol) propionic acid (mol wt 2 or 5 kDa, NHS-PEG-OCH3, Jenkem Technology Co. Ltd., Beijing, China) in HEPES-NaCl buffer (10 mM HEPES, 150 mM NaCl, pH 7.2) for 1 h. |
金蟲 (著名寫手)
| 2.7. 通過(guò)光波導(dǎo)模式譜(OWLS)的血清吸附試驗(yàn)。 通過(guò)光波導(dǎo)模式譜在未修飾和各種修飾的PHBV膜上進(jìn)行了非特異性蛋白由血清的吸附試驗(yàn)。30,33總而言之, 2wt%的PHBV旋涂到波導(dǎo)芯片上,并按2.1節(jié)中的敘述進(jìn)行隨后的表面修飾。為與MAL-PEG接枝的PHBV膜的性質(zhì)進(jìn)行比較,通過(guò)NH3-等離子體處理過(guò)的PHBV與甲氧基聚(乙二醇)丙酸(分子量2 or 5 kDa NHS-PEG-OCH3, Jenkem技術(shù)公司,中國(guó),北京)的2mM的N-羥基琥珀酰亞胺酯于HEPES - NaCl緩沖液(10mM HEPES, 150mM NaCl, pH7.2)中進(jìn)行反應(yīng)1小時(shí)制備了CH3OPEG-接枝的PHBV膜(PHBV-PEGOCH3)。 |
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