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malj09新蟲 (小有名氣)
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[交流]
藥理學(xué)高通量篩選中的一些詞匯----當合成遇到藥理的時候痛苦就來了，，，，急求賜教
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The data from six HTS campaigns were selected for analysis (SI Table S2). All HTS campaigns discussed in this paper displayed an acceptable signal-to-noise ratio as judged by the Z0 values 17 being greater than 0.5 in each assay event. The six HTS campaigns we selected employed AlphaScreen technology (PerkinElmer), which we have found to be particularly robust and reproducible. In these campaigns, rigorous compound triaging through counter- screens, secondary screens, and finally direct binding assess- ment by surface plasmon resonance is routinely undertaken. However, for the purposes of this current study, we delibe- rately selected the primary hit set (single point inhibition) as that which represented the least-processed set for each assay. Rather, we controlled for experimental aberration through the use ofmultiple (six) sets of primary screening hits. Primary hit selection criteria are given in SI Table S2.We interrogated the primary screening hits as follows. All library compounds were binned according to the number of assays in which they registered as hits according to those criteria for each particular assay. As a general rule, compound screening concentrations of between 10 and 30 μM were deployed. The results are shown in Table 2 |
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