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金陵棠邑新蟲 (小有名氣)
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[求助]
求教MES緩沖液如何配制? 已有1人參與
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| 請問PH=5.8,100mmol/L MES緩沖液如何配制?配制好的MES緩沖液能不能經(jīng)過高溫滅菌?如果不能,應(yīng)該怎樣利用其配制MS培養(yǎng)基? |
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Say you want to make 1 L of 100 mM MES at pH 6.0. The MW of MES (free acid) is 195.2. 1 L at 0.1 mole/L requires 0.1 mole of MES or 19.5 g of MES. Put 19.5 g of MES in something like 500 ml of water, put in a pH electrode (the starting pH will be something below 4.0 probably) and titrate to pH 6.0 with either 1 N or 10 N NaOH. Once you’ve got the solution to the correct pH, transfer it to a graduated cylinder or volumetric flask and dilute it to a final volume of 1 L and you’re done. Different volumes or different desired concentrations of MES will require the use of different amounts os solid MES, but the process will be the same. 這里有詳細的解答關(guān)于如何配置100mmol/L MES緩沖液如何配制?,配置好后可以高壓高溫滅菌! |
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