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月光寶盒7575新蟲(chóng) (初入文壇)
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[求助]
三親本雜交 熒光標(biāo)記質(zhì)粒
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看到一片文獻(xiàn),利用三親本雜交對(duì)質(zhì)粒熒光標(biāo)記,可是不理解:將質(zhì)粒pZP201-gfp轉(zhuǎn)移到Bacillus latersporus DSP (質(zhì)粒pDOC)中,就能將gfp片段連接到質(zhì)粒pDOC上,形成pDOC-gfp? 原理是什么? 這種方法(三親)與用酶切克隆gfp標(biāo)記有啥異同?怎么能保證成功?有沒(méi)有相關(guān)文獻(xiàn)可以借鑒? donor :E.Coli DH5α (質(zhì)粒pZP201-gfp) help: E.Coli HB101((質(zhì)粒 prk2013) recipient: Bacillus latersporus DSP (質(zhì)粒pDOC) The insertion of gfp into the pDOC plasmid was performed by tri- parental mating in which the Escherichia coli HB101 helper strain was used to mobilize the gfp segment from the Escherichia coli DH5a donor strain into the DSP recipient strain. <Plasmid-mediated bioaugmentation for the degradation of chlorpyrifos in soil> |
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