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疊瓣重華木蟲(chóng) (小有名氣)
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[求助]
做的有關(guān)酶固定化方向課題,最近投稿后審稿人的問(wèn)題我不太懂,希望大神幫忙。! 已有1人參與
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1. The authors could consider other parameters for studying the affinity of the enzyme for the substrate. They did the comparation with the free enzyme and immobilized enzyme for one substrate. The Michaeliana Kinetic is valid specially when the comparation is between different enzymes in front of one substrate. 本人的課題屬于交叉學(xué)科的,對(duì)酶這方面不是特別的熟,我之前看的文獻(xiàn)里表達(dá)酶和底物的affinity的用的都是米氏常數(shù)啊,審稿人讓我考慮用其他Parameters來(lái)研究酶和底物的親和力,我都不知道還有什么,請(qǐng)大神指點(diǎn)一下~ -The biocatalyst immobilized can present diffusion effects. Then, the authors did not prove that the km value is really different on the figure 3. -Catalytic efficiency decreased after the immobilization process but the km value must be done for each biocatalyst. 剩下的這兩條我不太明白審稿人的意思,求指點(diǎn)啊求指點(diǎn)!! |
新蟲(chóng) (初入文壇)
新蟲(chóng) (初入文壇)
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