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vc小小子鐵桿木蟲 (著名寫手)
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[求助]
摘要翻譯
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摘要： 為了研究鼠傷寒沙門菌crp缺失株對(duì)HeLa細(xì)胞黏附和侵入的影響，本試驗(yàn)對(duì)培養(yǎng)好的鼠傷寒沙門菌SL1344和SL1344Δcrp株進(jìn)行連續(xù)稀釋，隨后涂板計(jì)數(shù)，計(jì)算原菌液的CFU；對(duì)培養(yǎng)好的HeLa細(xì)胞調(diào)至每孔4×105 CFU，然后接種于6孔板中培養(yǎng)。將處理好的細(xì)菌懸液以感染復(fù)數(shù)（MOI）100:1加入到6孔板中，培養(yǎng)2小時(shí)，PBS洗滌后用胰酶消化，然后取細(xì)胞懸液100 μL連續(xù)10倍稀釋，挑選3個(gè)稀釋度進(jìn)行涂板計(jì)數(shù)，計(jì)算黏附率；.而侵入試驗(yàn)是在加入6孔板培養(yǎng)2 h后，用含10%血清和100 μg/mL的慶大霉素的DMEM培養(yǎng)基培養(yǎng)培養(yǎng)1.5 h，然后用裂解液處理該細(xì)胞，接著進(jìn)行對(duì)比稀釋，挑選3個(gè)梯度進(jìn)行涂板計(jì)數(shù)，計(jì)算侵入率。試驗(yàn)結(jié)果顯示，在黏附試驗(yàn)中，SL1344菌株的黏附率為0.155%，SL1344Δcrp株的黏附率為0.12%，差異顯著（P<0.05）。在侵入試驗(yàn)中，SL1344菌株的侵入率為0.1%，而SL1344Δcrp的侵入率為0.08%，兩者的侵入率相比差異顯著（P<0.05）。結(jié)果表明，crp缺失株對(duì)HeLa細(xì)胞的黏附率與侵襲率均發(fā)生降低，表明crp缺失株的致病力發(fā)生了減弱，本試驗(yàn)為研制安全有效的鼠傷寒沙門菌疫苗以及疫苗載體奠定了基礎(chǔ)。 關(guān)鍵詞：鼠傷寒沙門菌，crp，黏附，侵入 |
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燦燦妹
至尊木蟲 (著名寫手)
- 翻譯EPI: 68
- 應(yīng)助: 135 (高中生)
- 貴賓: 1.097
- 金幣: 14467.1
- 散金: 3921
- 紅花: 102
- 沙發(fā): 3
- 帖子: 2121
- 在線: 396.8小時(shí)
- 蟲號(hào): 1783324
- 注冊(cè): 2012-04-27
- 性別: GG
- 專業(yè): 環(huán)境工程
【答案】應(yīng)助回帖
| For investigating the effects of Salmonella typhimurium crp nullmutant to the adhesion and invasion of HeLa cell,two cultured strains (namely SL1344 and SL1344Δcrp) of Salmonella typhimurium were continuous diluted, followed by coated plates to calculate CFU of the original bacterium. |
2樓2015-05-31 08:50:51
燦燦妹
至尊木蟲 (著名寫手)
- 翻譯EPI: 68
- 應(yīng)助: 135 (高中生)
- 貴賓: 1.097
- 金幣: 14467.1
- 散金: 3921
- 紅花: 102
- 沙發(fā): 3
- 帖子: 2121
- 在線: 396.8小時(shí)
- 蟲號(hào): 1783324
- 注冊(cè): 2012-04-27
- 性別: GG
- 專業(yè): 環(huán)境工程
【答案】應(yīng)助回帖
| The cultured HeLa cells were adjusted to 4×105 CFU per hole, then were inoculated into 6-well plate, where the treated bacterial suspension was added into soon afterwards , as MOI was 100:1. After 2h cultivation, the 6-well plate was washed by PBS and digested by trypsin to fetch 100 μL bacterial suspension, which was continuous diluted 10 times, and 3 dilution were selected to coated plate count to calculate adhesion rate. While for invasion test, the 6-well plate were cultivated again by DMEM culture medium, whcih was composed by 10% serum and 100 μg/mL gentamicin, after 2h cultivation. And then the cell was treated by lysate, followed by compared dilution to select 3 dilution,which were used for coated plate counting to calculate invasion efficiency. |
3樓2015-05-31 09:22:39
燦燦妹
至尊木蟲 (著名寫手)
- 翻譯EPI: 68
- 應(yīng)助: 135 (高中生)
- 貴賓: 1.097
- 金幣: 14467.1
- 散金: 3921
- 紅花: 102
- 沙發(fā): 3
- 帖子: 2121
- 在線: 396.8小時(shí)
- 蟲號(hào): 1783324
- 注冊(cè): 2012-04-27
- 性別: GG
- 專業(yè): 環(huán)境工程
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
vc小小子: 金幣+35, ★★★很有幫助 2015-06-02 11:22:33
vc小小子: 金幣+35, ★★★很有幫助 2015-06-02 11:22:33
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Experimental results showed that, for adhesion test, the adhesion rate of SL1344 and SL1344Δcrp were 0.155% and 0.12% respectively, with significant difference （P<0.05）. While for invasion experiment, its invasion efficiency wer 0.1% and 0.08% separately, with significant different （P<0.05）. The results showed that adhesion rate and invasion efficiency of crp nullmutant to HeLa cell were degradation, which indicated that its virulence has weaken. This experiment has established the base of developing safe and effective Salmonella typhimurium vaccine and vaccine vector. Key words: Salmonella typhimurium, crp, adhesion, invasion |
4樓2015-05-31 09:38:54
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