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shaishaisong木蟲 (正式寫手)
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[求助]
請(qǐng)教各位大神如何從秀麗隱桿線蟲中提取脂質(zhì) 已有1人參與
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| 請(qǐng)教各位大神如何從秀麗隱桿線蟲中提取脂質(zhì) |
金蟲 (正式寫手)
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可以讀讀這篇protocol: Isolating lipid droplets from multiple species http://www.nature.com/nprot/journal/v8/n1/abs/nprot.2012.142.html |
金蟲 (正式寫手)
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The Caenorhabditis elegans lipidome: A primer for lipid analysis in Caenorhabditis elegans http://www.sciencedirect.com/sci ... i/S0003986115002672 C. elegans Major Fats Are Stored in Vesicles Distinct from Lysosome-Related Organelles http://www.sciencedirect.com/sci ... i/S1550413109003015 |
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本帖內(nèi)容被屏蔽 |
金蟲 (正式寫手)
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"To count worms - just dilute your sample and then count the number using a stereo microscope and then extrapolate the number of your original sample." For example, if you counted 100 larvae in 1 μl and have 10 ml of larvae, then you have a total of 100 × 10,000 =1,000,000 larvae. 可以參看下面的protocols High-throughput fluorescence-based isolation of live C. elegans larvae http://www.nature.com/nprot/journal/v7/n8/abs/nprot.2012.084.html |
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