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Ectoine and hydroxyectoine are well-recognized members of the compatible solutes and are widely employed by microorganisms as osmostress protectants. The EctABC enzymes catalyze the synthesis of ectoine from the precursor L-aspartate-b-semialdehyde. A subgroup of the ectoine producers can convert ectoine into 5-hydroxyectoine through aregion-selective and stereospecific hydroxylation reaction. Thiscompatible solute possessesstress-protective and function-preserving propertiesdifferent from thoseof ectoine.Hydroxylation of ectoineiscarried out by the EctDprotein,amember of the non-heme-containing iron (II) and 2-oxoglutarate-dependent dioxygenase superfamily. We used the signatureenzymesfor ectoine(EctC)and hydroxyectoine(EctD)synthesisin databasesearchesto assessthetaxonomicdistribution of potential ectoine and hydroxyectoine producers. Among 6428 microbial genomes inspected, 440 species are predicted to produce ectoine and of these, 272 are predicted to synthesize hydroxyectoine as well. Ectoine and hydroxyectoine genes are found almost exclusively in Bacteria. |
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Ectoine and hydroxyectoine are well-recognized members of the compatible solutes and are widely employed by microorganisms as posmostress protectants. 四氫嘧啶和羥基四氫嘧啶是公認(rèn)的相容性溶質(zhì),并廣泛運用于微生物領(lǐng)域,作為高滲脅迫保護劑。 The EctABC enzymes catalyze the synthesis of ectoine from the precursor L-aspartate-b-semialdehyde. A subgroup of the ectoine producers can convert ectoine into 5-hydroxyectoine through aregion-selective and stereospecific hydroxylation reaction. EctABC酶能催化,從前驅(qū)體左旋天冬氨酸b-半醛中合成四氫嘧啶。 有一個四氫嘧啶生產(chǎn)者的小組能通過一種具有區(qū)域指向性和立體定向性的羥基化反應(yīng),將四氫嘧啶轉(zhuǎn)變成5-羥基四氫嘧啶。 This compatible solute possessesstress-protective and function-preserving properties different from those of ectoine.Hydroxylation of ectoine is carried out by the EctD protein,a member of the non-heme-containing iron (II) and 2-oxoglutarate-dependent dioxygenase superfamily. 這種相容性溶質(zhì),具有不用于四氫嘧啶的,壓力保護和功能保留的特性。通過加入EctD蛋白質(zhì)這種屬于非血紅素鐵和酮戊二酸的雙加氧酶家族的物質(zhì),能夠催化四氫嘧啶的羥基化反應(yīng)。 We used the signature enzymes for ectoine(EctC)and hydroxyectoine(EctD)synthesis in database searches to assess the taxonomic distribution of potential ectoine and hydroxyectoine producers. 我們運用標(biāo)記酶來催化EctC(四氫嘧啶)和EctD(羥基四氫嘧啶)的合成反應(yīng),在數(shù)據(jù)庫檢索,評估出潛在的EctC和EctD生產(chǎn)者的分類分布。 Among 6428 microbial genomes inspected, 440 species are predicted to produce ectoine and of these, 272 are predicted to synthesize hydroxyectoine as well. Ectoine and hydroxyectoine genes are found almost exclusively in Bacteria. 在一共調(diào)查的6428個微生物基因組中,有440種被認(rèn)為是可以生產(chǎn)四氫嘧啶的,而在這440鐘潛在生產(chǎn)者中,又有272種被認(rèn)為可以生產(chǎn)羥基四氫嘧啶。四氫嘧啶和羥基四氫嘧啶基因,幾乎只在細(xì)菌中有被發(fā)現(xiàn)過。 |
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