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Ectoine belongs to the family of compatible solutes, which are known to contribute mainly to the adaptation of the cell to osmotic stress by mediation of a constant turgor. In addition, the cell’s essential functions are maintained under difficult conditions like high salinity, heat, or aridity stress. Biosynthesis of ectoine has been found in halophilic and halotolerant microorganisms. We showed that the methanotrophic bacterial consortium (MBC) isolated from coalbed rocks from coalmine Bogdanka (Poland) and resistant to extreme environmental conditions (low content of moisture) was able to synthesize ectoine. MBC was cultured in mineral nitrate mineral salts medium supplied with NaCl at atmospheric air enriched with 10% of methane. The levels of methanotrophic activity were determined by the gas chromatography technique (943.05 ± 30.73 – 94.14 ± 0.85 μ M CH 4 gDW –1 day –1 ) and the biomass concentration of MBC was evaluated based on OD 600 ,as well as biosynthesis of ectoine in relation to the salinity (0–5% NaCl) of the medium. The levels of ectoine tested by NIR measurements ranged from 1.33 ± 0.10 mg gDW –1 to 0.42 ± 0.08 mg gDW –1 depending on the salinity of the solution. In addition, we identified MBC based on the pmo A gene. |
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至尊木蟲 (著名寫手)
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【答案】應(yīng)助回帖
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bxzc123: 金幣+35, 翻譯EPI+1, ★★★★★最佳答案 2015-06-12 10:19:54
bxzc123: 金幣+35, 翻譯EPI+1, ★★★★★最佳答案 2015-06-12 10:19:54
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四氫嘧啶屬于可混溶溶質(zhì)。已知這類溶質(zhì)在細(xì)胞以恒定膨壓適應(yīng)滲透應(yīng)力時起主要作用。此外，在生存困境如高鹽、熱或干旱脅迫時細(xì)胞的基本功能得以維持。已發(fā)現(xiàn)嗜鹽和耐鹽的微生物可生物合成四氫嘧啶。我們發(fā)現(xiàn)從波格丹卡（波蘭）煤礦的煤床巖層分離的甲烷營養(yǎng)菌群（MBC)能抗極端環(huán)境條件（低濕度），能合成四氫嘧啶。將MBC用添加NaCl的礦物硝酸鹽礦物鹽培養(yǎng)基于富含10%甲烷的氣體下培養(yǎng)。甲烷營養(yǎng)活性用氣相色譜法測定（943.05 ± 30.73 – 94.14 ± 0.85 μ M 甲烷/克干樣/天),MBC的生物量濃度用OD 600表示，并確定了四氫嘧啶生物合成與培養(yǎng)基中鹽濃度（0-5%NaCl)的關(guān)系。四氫嘧啶濃度用近紅外法測定為 1.33 ± 0.10 毫克/克干樣 至 0.42 ± 0.08 毫克/克干樣，取決于溶液中鹽濃度。 另外，我們還以特異甲烷加單氧酶A 鑒定MBC. |
2樓2015-06-11 23:30:37
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