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[求助]
LC-MS/MS表征是否某真菌產(chǎn)生了果膠酶,所需要測(cè)的數(shù)據(jù)都包括哪些? 已有2人參與
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本人準(zhǔn)研一小碩一枚,導(dǎo)師讓我做驗(yàn)證一個(gè)真菌是否產(chǎn)果膠酶的LC-MS/MS 的實(shí)驗(yàn),不知道LC-MS/MS 表征這一結(jié)果的數(shù)據(jù)都包括什么,具體思路是什么,求前輩們指教! 具體情況是投的文章收到了修改的要求,如下文。 Reviewer #4: The authors revised the manuscript carefully accoring to the reviewers, and the quality of this version was greatly improved. However, as pointed out before that the authors should utilize a proteomic approach to verify their findings, only this result could conform the puried band is the polygalacturonase secreted by A.fumigatus M67.Regrettably, they never show this result. The manuscript can not be accepted except providing the LC-MS/MS data of this band. 不勝感激! |
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