 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 759 | 回復(fù): 4 | ||
喵個叁叁銅蟲 (初入文壇)
|
[求助]
求翻譯�。�！
|
|
Trace Zn2+ and Cu2+ in living cells play important roles in the regulation of biological function. It is significant to simultaneously detect the cellular Zn2+ and Cu2+. Here, we present a novel two-color fluorescence nanoprobe based on the DNAzymes for simultaneous imaging of Zn2+ and Cu2+ in living cells. The probe consists of a 13 nm gold nanoparticle, DNAzymes that are specific for Zn2+ and Cu2+, and the substrate strands labeled with fluorophores at the 5′ end and quenchers at the 3′ end. The fluorescence of the fluoreophores is quenched both by the gold nanoparticle and the quencher. After the nanoprobes are transferred into the cells, the substrate strands would be cleaved in the presence of the Zn2+ and Cu2+ target, resulting in disassociation of the shorter DNA fragments containing fluorophores, which produce fluorescence signals correlated with the location and concentration of the Zn2+ and Cu2+. The nanoprobe exhibits high specificity, nuclease stability, and good biocompatibility. Moreover, the nanoprobe can simultaneously monitor the cellular Zn2+ and Cu2+ with an on-site manner, providing the information on localization and concentration of targets, which is significant to further research the Zn2+- and Cu2+-relative cellular events and biological process. The proposed method has shown great potential in the detection of multiple metal ions in living cells, which may help us to better understand the function of metal ions in the fields of biochemistry, molecular biology, and cellular toxicology. 在線等!!! |
回復(fù)此樓» 猜你喜歡
生物學(xué)學(xué)碩，一志愿湖南大學(xué)，初試成績338
已經(jīng)有5人回復(fù)
-
332求調(diào)劑
已經(jīng)有3人回復(fù)
-
343求調(diào)劑
已經(jīng)有3人回復(fù)
-
學(xué)碩274求調(diào)劑
已經(jīng)有8人回復(fù)
-
壓國家一區(qū)線，求導(dǎo)師收留，有恩必謝！
已經(jīng)有7人回復(fù)
-
081200-11408-276學(xué)碩求調(diào)劑
已經(jīng)有4人回復(fù)
-
22408 359分調(diào)劑
已經(jīng)有4人回復(fù)
-
本科新能源科學(xué)與工程，一志愿華理能動285求調(diào)劑
已經(jīng)有5人回復(fù)
-
一志愿華北電力大學(xué)能動專碩，293，求調(diào)劑
已經(jīng)有5人回復(fù)
-
調(diào)劑310
已經(jīng)有7人回復(fù)
喵個叁叁
銅蟲 (初入文壇)
- 應(yīng)助: 4 (幼兒園)
- 金幣: 196
- 帖子: 49
- 在線: 18.3小時
- 蟲號: 3709214
- 注冊: 2015-03-04
- 專業(yè): 微生物學(xué)
2樓2015-06-14 16:49:52
fjtony163
版主 (文壇精英)
米米
- 翻譯EPI: 386
- 應(yīng)助: 361 (碩士)
- 貴賓: 6.876
- 金幣: 70610.6
- 散金: 36050
- 紅花: 222
- 沙發(fā): 616
- 帖子: 21912
- 在線: 3793.7小時
- 蟲號: 1397876
- 注冊: 2011-09-11
- 專業(yè): 遺傳學(xué)研究新技術(shù)與方法
- 管轄: 論文翻譯
3樓2015-06-14 19:03:30
krispanlove
金蟲 (小有名氣)
- 應(yīng)助: 26 (小學(xué)生)
- 金幣: 719.4
- 紅花: 21
- 帖子: 254
- 在線: 29.1小時
- 蟲號: 3898944
- 注冊: 2015-05-30
- 性別: MM
- 專業(yè): 環(huán)境工程
4樓2015-06-18 16:37:59
Frieda2014
新蟲 (初入文壇)
- 翻譯EPI: 1
- 應(yīng)助: 0 (幼兒園)
- 金幣: 11.5
- 紅花: 1
- 帖子: 35
- 在線: 12.1小時
- 蟲號: 3654289
- 注冊: 2015-01-20
- 專業(yè): 藥物分析
【答案】應(yīng)助回帖
| 恒量的Zn2+和Cu2+對活細(xì)胞的生理功能調(diào)節(jié)起重要作用。因此同時測定細(xì)胞中的Zn2+和Cu2+很有必要。本文，我們采用新型雙色熒光探針基于DNA酶同時對活細(xì)胞中的Zn2+和Cu2+成像。該探針由13nm金納米粒，特定的Zn2+和Cu2+DNA酶，及5′端用熒光基團標(biāo)記、3′端用淬滅劑標(biāo)記的底物鏈組成。熒光基團的熒光性同時被金納米粒和淬滅劑淬滅。熒光探針進去細(xì)胞之后，底物鏈會由于的Zn2+和Cu2+靶標(biāo)的存在而斷裂，導(dǎo)致含熒光探針的DNA短片段的解離，從而產(chǎn)生了與Zn2+和Cu2+濃度及位置相關(guān)的熒光信號。納米探針展現(xiàn)了高特異性、核酸酶穩(wěn)定性及良好的生物相容性。此外，納米探針可實時實地地對細(xì)胞內(nèi)的Zn2+和Cu2+進行監(jiān)測，從而提供靶標(biāo)位置及濃度的相關(guān)信息，對進一步研究與Zn2+和Cu2+相關(guān)的細(xì)胞內(nèi)物質(zhì)及生理反應(yīng)非常重要。本文提出的方法，可對活細(xì)胞中多金屬離子檢測，從而幫助我們更好地了解金屬離子在生物化學(xué)、分子生物學(xué)、細(xì)胞毒理學(xué)中的作用 |
5樓2015-07-07 12:29:56
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 喵個叁叁 的主題更新
| 5 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 一志愿北化085600材料專碩275|有文章專利｜求調(diào)劑 +5 | Micky11223 2026-03-25 | 5/250 |
|
|---|---|---|---|---|
|
[考研] 321求調(diào)劑 +6 | 材料cailiao 2026-03-21 | 6/300 |
|
|
|
[考研] 求調(diào)劑 +6 | 蘆lty 2026-03-25 | 7/350 |
|
|
|
[考研] 0703一志愿9，初試成績：338，四六級已過，有科研經(jīng)歷，求調(diào)劑！ +4 | Zuhui0306 2026-03-25 | 4/200 |
|
|
|
[考研] 085701環(huán)境工程，267求調(diào)劑 +16 | minht 2026-03-26 | 16/800 |
|
|
|
[考研] 0703化學(xué)/290求調(diào)劑/本科經(jīng)歷豐富/工科也可 +9 | 丹青奶蓋 2026-03-26 | 10/500 |
|
|
|
[考研] 張芳銘-中國農(nóng)業(yè)大學(xué)-環(huán)境工程專碩-298 +4 | 手機用戶 2026-03-26 | 4/200 |
|
|
|
[考研] 一志愿鄭大085600，310分求調(diào)劑 +5 | 李瀟可 2026-03-26 | 5/250 |
|
|
|
[考研] 292求調(diào)劑 +4 | 求求了收下我吧?/a> 2026-03-26 | 4/200 |
|
|
|
[考研]
 304材料求調(diào)劑
+4
|
鐘llll 2026-03-26 | 4/200 |
|
|
|
[考研] 336材料求調(diào)劑 +7 | 陳瀅瑩 2026-03-26 | 9/450 |
|
|
|
[考研] 生物學(xué) 296 求調(diào)劑 +4 | 朵朵- 2026-03-26 | 6/300 |
|
|
|
[考研]
085602 289分求調(diào)劑
+8
|
WWW西西弗斯 2026-03-24 | 8/400 |
|
|
|
[考研] 290分調(diào)劑求助 +3 | 吉祥止止陳 2026-03-25 | 3/150 |
|
|
|
[考研] 考研一志愿蘇州大學(xué)初始315（英一）求調(diào)劑 +3 | sbdksD 2026-03-24 | 4/200 |
|
|
|
[有機交流]
有機合成求助
 20+3
|
FENGSHUJEI 2026-03-23 | 5/250 |
|
|
|
[考研] 292求調(diào)劑 +4 | 鵝鵝鵝額額額額?/a> 2026-03-24 | 4/200 |
|
|
|
[考研] 361求調(diào)劑 +3 | Glack 2026-03-22 | 3/150 |
|
|
|
[考研] 接收2026碩士調(diào)劑(學(xué)碩+專碩) +4 | allen-yin 2026-03-23 | 6/300 |
|
|
|
[考研] 070300，一志愿北航320求調(diào)劑 +3 | Jerry0216 2026-03-22 | 5/250 |
|