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[求助]
英譯漢
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In mitochondrion, a small portion (about 15%) of cyt c is tightly bound to the membrane while the remainder is free or loosely bound [1,2]. The unbound cyt c participates in eT and actively prevents oxidative stress,while membrane-bound cyt c ismainly involved in cell death. To initiate the apoptotic process the protein acquires peroxidase activity, an event which favors protein dissociation from the mitochondrial membrane [9,39,40] and the subsequent release of cyt c into the cytosol. Among the phospholipids constituting the mitochondrial membrane, CL is known to bind the hemoprotein [41]. Thus, the cyt c–CL interaction plays an important role in cells since it modulates the protein behavior determining whether cyt c should perform its ‘normal’ role in the respiratory chain or, conversely, is to be released into the cytosol where it participates in the apoptotic event (see [40] and references therein). Unlike the equine protein, cyt c fromyeast does not participate in cell apoptosis [16,17]. In this study we investigated the interaction of horse and yeast cyt c with CL vesicles, here considered a model for the CL-containing mitochondrial membrane, with the aim of identifying the factors distinguishing equine and yeast cyt c in their reaction with CL liposomes. The present data clearly show that only the horse cyt c–CL interaction is significantly affected by the ionic strength and ATP (both of which also exert considerable influence on the complex stability). Therefore,when the CL–cyt c binding reaction is investigated, it must be considered that c-type cyts purified from different sources may display different behavior. |
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★
愛(ài)與雨下: 金幣+1 2015-07-03 20:46:18
不辜負(fù): 金幣+5, 翻譯EPI+1, ★★★很有幫助, 謝謝你噢 2015-07-08 01:19:58
愛(ài)與雨下: 金幣+1 2015-07-03 20:46:18
不辜負(fù): 金幣+5, 翻譯EPI+1, ★★★很有幫助, 謝謝你噢 2015-07-08 01:19:58
|
In mitochondrion, a small portion (about 15%) of cyt c is tightly bound to the membrane while the remainder is free or loosely bound [1,2]. The unbound cyt c participates in eT and actively prevents oxidative stress,while membrane-bound cyt c ismainly involved in cell death. To initiate the apoptotic process the protein acquires peroxidase activity, an event which favors protein dissociation from the mitochondrial membrane [9,39,40] and the subsequent release of cyt c into the cytosol. Among the phospholipids constituting the mitochondrial membrane, CL is known to bind the hemoprotein [41]. Thus, the cyt c–CL interaction plays an important role in cells since it modulates the protein behavior determining whether cyt c should perform its ‘normal’ role in the respiratory chain or, conversely, is to be released into the cytosol where it participates in the apoptotic event (see [40] and references therein). Unlike the equine protein, cyt c fromyeast does not participate in cell apoptosis [16,17]. 在線粒體中，少部分（約 15%）cyt c 緊密結(jié)合于膜上，而其他的是游離的或松散地結(jié)合的 [1,2]. 未結(jié)合的 cyt c 參與 eT，積極地防止氧化脅迫，而膜結(jié)合的 cyt c 主要參與細(xì)胞死亡。 要開(kāi)始細(xì)胞凋亡過(guò)程， 蛋白獲得過(guò)氧化物酶活性，這一事件促進(jìn)蛋白從線粒體膜上解離 [9,39,40]，以及隨后的 cyt c 釋放如胞質(zhì)。i在組成線粒體膜的磷脂中，已知 CL 結(jié)合血紅素蛋白[41]。 因此，cyt c–CL 相互作用在細(xì)胞中有重要功能，因?yàn)樗{(diào)控蛋白行為，決定cyt c 在呼吸鏈中行使“正�！惫δ埽蛘呦敕�，被釋放到胞質(zhì)中參與凋亡事件 (參見(jiàn) [40] 及其參考文獻(xiàn)). 與馬中的蛋白不同，來(lái)自酵母的 cyt c 不參與細(xì)胞凋亡 [16,17]. |
3樓2015-07-03 13:52:47
krispanlove
金蟲 (小有名氣)
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2樓2015-06-23 16:18:38
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