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| A standard β-sitosterol sample was procured from Fluka Chemicals and analyzed at the laboratory by gas chromatography (GC). Fish oil (Mega-Shelcal capsules from Elder Pharmaceuticals, India) was used as the source of eicosapentanoic acid (EPA) and docosahexaenoic acid (DHA), and the GC analysis of the fish oil showed that the oil contained 32 % EPA and 22 % DHA. Refined, bleached flaxseed oil procured from V.K.V.K. Oil Limited, Kolkata, India, was used as the source of alpha linolenic acid (ALA), and the GC analysis of the oil showed the presence of 54 % ALA in the oil. Thermomyces lanuginosus lipase (Lipozyme TLIM) used as biocatalyst, was a generous gift from Novozyme India, Ltd., Bangalore, India. Phytosterol esters were formed by enzymatic transesterification reactions in a packed bed reactor and their fatty acid compositions were analyzed by GC (Sengupta et al. 2010). The yield of phytosterol esters were 95 % EPA-DHA rich sterol ester and 95.2 % ALA rich sterol ester. The sterol esters were purified using column chromatography. |
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2樓2015-08-03 15:29:02
3樓2015-08-04 09:22:32
baoshanqiu
至尊木蟲 (著名寫手)
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
小丸丸9: 金幣+25, 翻譯EPI+1, ★★★★★最佳答案, 謝謝 2015-08-05 14:20:24
小丸丸9: 金幣+25, 翻譯EPI+1, ★★★★★最佳答案, 謝謝 2015-08-05 14:20:24
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標(biāo)準(zhǔn)β-谷甾醇樣品從Fluka化學(xué)試劑公司獲得，實驗室用氣相色譜法（GC）進行分析。魚油（來自于印度Elder藥劑公司的巨型Shelcal 膠囊）作為二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的來源，用GC分析魚油表明該油含32%EPA和22%DHA。精煉、漂白的亞麻籽油從印度加爾各答VKVK油料有限公司獲得，用作α-亞麻酸(ALA)的來源，GC分析表明該油含54%ALA。疏棉狀嗜熱絲孢菌酯酶（酯酶 TLIM)作為生物催化劑，由印度班加羅爾Novozyme India, Ltd慷慨惠贈。植物甾醇酯是在填料床反應(yīng)器內(nèi)用酶轉(zhuǎn)酯反應(yīng)合成，其脂肪酸組成用GC分析（Sengupta 等. 2010)。植物甾醇酯的得率為95%富含EPA-DHA甾醇脂和95.2%富含ALA甾醇脂。這些甾醇脂用柱色譜純化。 從這段文字看，沒有使用EPA-DHA phytosterol ester，而是合成的phytosterol ester富含EPA-DHA，也沒說具體含多少。從原文結(jié)果表1中可見C20：5含量為39.20+-1.10%，C22：6含量為31.98+-1.63%。 |
4樓2015-08-04 23:01:59
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