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轉(zhuǎn)錄組里差異性表達的轉(zhuǎn)錄因子不同家族的分析
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hello everyone, i have finished differential expression analysis of my RNA-seq data and retrieve the transcripts of transcription factors(TF) from differentially expressed unigenes and non differentially expressed unigenes. then i have categorized those TFs to different TF families. then i realized there are many TFs sequences which blong to the same TF family overly expressed than some other TF families, some parts of my data: TF_families Differential Unchanged AP-2 0 2 ARID 2 5 bHLH 8 67 CG-1 1 1 COE 0 2 CP2 0 4 CSD 0 4 CSL 0 1 CUT 1 6 DDT 0 1 DM 0 6 E2F 0 3 ETS 2 10 Fork_head 2 22 . . . and i wanna find most expressed TF families in my DE TF sets. i saw a several papers analysed this kind of data and they used fisher's exact test for that. i searched some materials about fisher's exact test, but in those example fisher's test used for 2x2 contingency table, no more. here i still have no idea how to use fisher's test to my data. so could anyone explain to me how could i do solve this and is there any tool i can use for the analysis ? any suggestion would be highly appreciated. |
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