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oreYouBegin PrecautionsNLysis/BindingBufferandWashBufferIcontainguanidinehydrochloride whichisanirritant.Alwayswearglovesandfollowstandardsafetypre- cautionstominimizecontactwhenhandling. ?Donotletthesebufferstouchyourskin,eyes,ormucousmembranes.If contactdoesoccur,washtheaffectedareaimmediatelywithlargeamounts ofwater;otherwise,thereagentmaycauseburns.Ifyouspillthereagent, dilutethespillwithwaterbeforewipingitup. ?NeverstoreorusetheBindingBuffernearhumanoranimalfood. ?Alwayswearglovesandfollowstandardsafetyprecautionswhenhandling thesebuffers. SampleMaterialSolidtissue(e.g.,mouseliver,spleen,lung,heart),1–10mg(formortar/pestle disruption)or1–25mg(forrotor-statorhomogenization). Handlingand Storageof StartingMaterial ToavoidanydegradationofRNAinsamplematerialbyintracellularRNases followtheseguidelines: ?Processthesamplematerialassoonascollected ?Ifstorageofsamplematerialisrequired,flashfreezeinliquidnitrogenand storeat-60°C NDonotallowfrozentissuematerialtothawduringhandling(e.g., weighing).Therelevantproceduresshouldbecarriedoutasquicklyas possible. ?Samplescanalsobestoredat-60°CorbelowinLysis/BindingBufferafter disruptionandhomogenization. LYieldsmayvarydependingonstoragetime. NUsesteriledisposablepolypropylenetubesandtipsinordertoavoid RNasecontamination.Wearglovesduringtheassay. Disruptionand Homogenization ?Efficientdisruptionandhomogenizationofthestartingmaterialisessential forintra-cellularRNAisolationproceduresfromtissues. ?Thecompletedisruptionofcellwallsandplasmamembranesofcellsand organellesisabsolutelyrequiredtoreleasealltheRNAcontainedinthesam- ple.Incompletedisruptionresultsinsignificantlyreducedyields. ?Homogenizationisnecessarytoreducetheviscosityofthecelllysatespro- ducedbydisruption.Homogenizationshearsthehighmolecularweight genomicDNAandotherhighmolecularweightcellularcomponentstocre- ateahomogeneouslysate. ?Incompletehomogenizationresultsinsignificantlyreducedyields.Somedis- ruptionmethodssimultaneouslyhomogenizethesample,whileothers requireanadditionalhomogenizationst 發(fā)自小木蟲Android客戶端 |
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使用前 【注意 】 .結(jié)合緩沖液和沖洗緩沖液I含有鹽酸胍,是一種刺激性物質(zhì)。實(shí)驗(yàn)全程戴手套,并遵循標(biāo)準(zhǔn)的安全防范措施,以盡量減少接觸。 .不要讓這些緩沖液接觸你的皮膚、眼睛或粘膜。如果接觸到,立即用大量的水清洗受污染的地區(qū);否則,該試劑會(huì)引起燒傷。如果試劑泄漏,在抹擦之前用水稀釋。 .在人或動(dòng)物食品的附近,不要儲(chǔ)存或使用結(jié)合緩沖液。 .在處理這些緩沖液時(shí),戴上手套,并遵循標(biāo)準(zhǔn)的安全防護(hù)措施。 【實(shí)驗(yàn)材料】 |
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