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[求助]
關于藥物溶解在DMSO中的稀釋問題
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| 買的試劑,共0.5mg,分子量650.75g/mol,試劑的工作濃度為1μM,自己是這樣算的,0.5mg的藥品全部溶解在10ml DMSO中,然后取出10μl 加到990μl DMSO 中,再然后取取出10μl 加到990μl DMSO 中,最后取13μl 加入到100ml 我的工作液中,這樣濃度剛好是1μM,但是后來看藥物介紹說這個藥溶于DMSO,但有這么一句話不明白什么意思:No initial concentrations of TG were given for either solvent but it was noted that the final concentrations were: in DMSO, less than 1% (v/v),ethanol, did not exceed 1% of the total volume (working concentrations in ethanol were from 70-140 mM).,這是什么意思,意思是在DMSO中溶解度很小嗎,那我把0.5mg的藥品全部溶解在10ml DMSO中是不是不行?有沒有懂的? |
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