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icebergwu木蟲之王 (文學泰斗)
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[求助]
求翻譯，分析方向
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| 利用超高效液相色譜-高分辨四極桿飛行時間質(zhì)譜對飼料中的抗生素5種組分進行鑒別分析。采用C18色譜柱分離，以乙腈-0.1%甲酸水溶液(含2mmol•L-1乙酸銨)為流動相梯度洗脫，流速0.4mL•min-1，柱溫40℃；質(zhì)譜條件為電噴霧離子源，檢測方式為負離子全掃描模式，通過保留時間、精確分子量和二級質(zhì)譜特征碎片完成對抗生素的5種組分的鑒別。與高效液相色譜方向比較，本方法具有判斷準確、檢測快速的特點，具有很好的應用價值。 |
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武漢一心一譯 
捐助貴賓 (著名寫手)
- 翻譯EPI: 502
- 應助: 8 (幼兒園)
- 金幣: 2283.1
- 散金: 5914
- 紅花: 32
- 帖子: 1665
- 在線: 321.9小時
- 蟲號: 3587652
- 注冊: 2014-12-10
- 性別: GG
- 專業(yè): 高分子合成化學
【答案】應助回帖
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icebergwu: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案 2015-11-20 11:08:24
icebergwu: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案 2015-11-20 11:08:24
| Using ultra-high performance liquid chromatography (UPLC)-high resolution quadrupole time-of flight MS, the discriminant analysis of 5 components of the feed was conducted. Based on C18 chromatogram column separation, the acetonitrile - 0.1% formic acid aqueous solution (including 2mmol•L-1 ammonium acetate) was employed as the mobile phase at flow rate of 0.4mL•min-1 for gradient elution at column temperature of 40℃; Under the mass spectrometer condition of electrospray ionization, and adopting anion full scan mode as detection mode, the identification of 5 components of the feed were implemented based on retention time, exact molecular weight, and secondary mass spectral characteristic fragments. Compared with HPLC, this method is of better application value for its advantages of accurate judgement and rapid detection. |
2樓2015-11-20 11:07:01
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