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chenglong9797木蟲 (著名寫手)
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[求助]
求助：英譯漢，一段摘要
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Recent advances in RNA nanotechnology allow the rational design of various nanoarchitectures Previous methods utilized conserved angles from natural RNA motifs to form geometries with specific sizes. However, the feasibility of producing RNA architecture with variable sizes using native motifs featuring fixed sizes and angles is limited. It would be advantageous to display RNA nanoparticles of diverse shape and size derived from a given primary sequence. Here, we report an approach to construct RNA nanoparticles with tunable size and stability. Multifunctional RNA squares with a 90° angle were constructed by tuning the 60° angle of the three-way junction (3WJ) motif from the packaging RNA (pRNA) of the bacteriophage phi29 DNA packaging motor. The physicochemical properties and size of the RNA square were also easily tuned by modulating the “core” strand and adjusting the length of the sides of the square via predictable design. Squares of 5, 10, and 20 nm were constructed, each showing diverse thermodynamic and chemical stabilities. Four “arms” extending from the corners of the square were used to incorporate siRNA, ribozyme, and fluorogenic RNA motifs. Unique intramolecular contact using the pre-existing intricacy of the 3WJ avoids relatively weaker intermolecular interactions via kissing loops or sticky ends. Utilizing the 3WJ motif, we have employed a modular design technique to construct variable-size RNA squares with controllable properties and functionalities for diverse and versatile applications with engineering, pharmaceutical, and medical potential. This technique for simple design to finely tune physicochemical properties adds a new angle to RNA nanotechnology. |
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disjustar
鐵桿木蟲 (正式寫手)
- 應(yīng)助: 1 (幼兒園)
- 金幣: 7421.4
- 散金: 300
- 紅花: 24
- 帖子: 999
- 在線: 302.9小時
- 蟲號: 2831845
- 注冊: 2013-11-27
- 性別: GG
- 專業(yè): 病原真菌學
2樓2015-12-09 11:19:01
jnxybsf
金蟲 (正式寫手)
- 翻譯EPI: 15
- 應(yīng)助: 0 (幼兒園)
- 金幣: 1305.4
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- 沙發(fā): 1
- 帖子: 582
- 在線: 81.8小時
- 蟲號: 2042949
- 注冊: 2012-10-04
- 專業(yè): 元素化學
【答案】應(yīng)助回帖
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chenglong9797: 金幣+40, 翻譯EPI+1, ★★★很有幫助, 謝謝�。�！ 2015-12-10 09:15:10
chenglong9797: 金幣+40, 翻譯EPI+1, ★★★很有幫助, 謝謝�。�！ 2015-12-10 09:15:10
| 納米技術(shù)的最新進展考慮到了不同納米建構(gòu)的合理設(shè)計。目前的方法利用天然RNA基序的保守角形成了特定尺寸的幾何構(gòu)型。人造RNA是由固定尺寸和角度的天然基序合成，而對于這些人造RNA不同尺寸的構(gòu)建，上述方法的使用是有限的。它有助于解釋RNA納米顆粒中一段給定主序列造成的不同形狀和尺寸。我們報道了一種用可調(diào)尺寸和穩(wěn)定性構(gòu)建RNA納米顆粒的方法。多功能RNAsquares中有個90度角，該角是通過調(diào)整蛋白基序中3WJ形成的60度角得到的，此蛋白基序取自噬菌體 phi29 DNA packaging motor的pRNA。調(diào)整這些“中心”束，并根據(jù)預定的設(shè)計改變square的邊長也可以容易地調(diào)整RNAsquare的物理化學性質(zhì)和尺寸。已得到邊長分別為5，10和20納米的Square(樣方？)，每一個樣方表現(xiàn)出不同的熱力學和化學穩(wěn)定性，從樣方的四個角延伸出的“鏈”被用來結(jié)合 siRNA, ribozyme和 fluorogenic RNA motifs。3WJ先前存在的復雜性形成了獨特的分子內(nèi)接觸，這就避免了由親環(huán)或粘性末端導致的相對較弱的分子內(nèi)反應(yīng)。我們已經(jīng)用3WJ基序通過一種分子設(shè)計技術(shù)組裝了具有可控性質(zhì)和功能的可變尺寸RNAsquare，它可被廣泛用于工程、制藥和醫(yī)療。通過簡單設(shè)計改變其物理化學質(zhì)，該技術(shù)為RNA納米技術(shù)帶來了新的視角。 外行 僅供參考 盡請指正 |
3樓2015-12-09 22:34:31
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