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[求助] 納米簇的分離

文獻(xiàn)是這樣說的：PAGE was performed with a Biorad Protean II XI system with gels of 3 mm thickness. The experimental
conditions for PAGE are close to those employed in previous works.3 The total content of the acrylamide
monomers were 3% (acrylamide/Bis, 94 : 6) and 25% (acrylamide/Bis, 93 : 7) for the stacking gel and
separation gel, respectively. The staking and the separating gels were buffered at pH = 6.8 and 8.8,
respectively, with Tris-HCl solution. The eluting buffer consisted of a solution of glycine (192 mM) and
Tris (25 mM) in 80 : 20 (v/v) water : methanol. The purified NPs were dissolved in a 5% glycerol solution
in water to a concentration of 4 mg.ml-1. The NPs solutions (2 ml) were loaded on a 3 mm gel without
lanes and eluted for 17 hours at a constant voltage of 150 V to achieve separation. Parts of the gel
containing each separated fraction were cut out and placed in Milli-Q water overnight. The gel lumps
suspended in the solution were removed by filtration. NPs were finally further purified by dialysis in the
same way as described above. For each ligand exchange reaction described below, 6 to 8 gels are necessary
in order to get sufficient quantities of size separated NPs.（原文：J. AM. CHEM. SOC. 2008, 130, 7077–7084 9 7077）

就是選用native-page 將不同粒徑的納米簇分開，然后再將膠里面的納米簇溶解；我做到分離納米簇以后想要將納米簇提取出來，切碎以后，用水溶解，發(fā)現(xiàn)提取不出來，是膠配的太大還是有其他操作步驟沒有注意到。請問有做過這一方面的專家么，能否給予小弟幫助，謝謝。@nowitzki_ci

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