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[求助]
陽離子聚合物的DLS水合粒徑與TEM粒徑不一致的問題 已有2人參與
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各位親們,文章審稿人提出問題,讓就“DLS水合粒徑與TEM粒徑不一致的問題”引用文獻(xiàn)多加解釋。我目前的理解是TEM測試下的復(fù)合粒子是已經(jīng)干燥的納米粒子,而DLS是溶液狀態(tài)下的復(fù)合粒子粒徑。粒子在水溶液中發(fā)生一定的溶脹現(xiàn)象,導(dǎo)致水合粒徑大于干燥粒子。 但是查閱了好久,并沒有找到直接給出解釋的文章。 不知道有沒有蟲友可以幫忙提供相關(guān)文獻(xiàn)的,非常感謝! |
鐵桿木蟲 (著名寫手)
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這是再正常不過的現(xiàn)象啊 researchgate上有一個人解釋的答案 DLS gives a hydrodynamic size that corresponds to the core and the swollen corona of the micelles, whereas TEM often gives a size of the core for micelles in a dried state as the corona with low electronic density is not visible. |
鐵桿木蟲 (正式寫手)
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However, the size was smaller than that measured by DLS. This was mainly due to the process involved in the preparation of sample. In the case of the TEM and SEM methods, TEM and SEM images depicted the actual size at the dried state of sample, whereas the size measured by the laser light scattering method was a hydrodynamic diameter (hydrated state), and therefore the nanoparticles showed a larger hydrodynamic volume due to solvent effect in the hydrated state. 來源:The potential of self-assembled, pH-responsive nanoparticles of mPEGylated peptide dendron–doxorubicin conjugates for cancer therapy |
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