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[資源]
如何完全裂解細(xì)胞?如何處理蛋白樣?
CST推薦WB操作流程中的關(guān)鍵步驟——這些步驟非常重要操作步驟的變化可對WB結(jié)果產(chǎn)生顯著影響。我們對裂解產(chǎn)物制備、凝膠、轉(zhuǎn)移、轉(zhuǎn)膜、緩沖液、封閉以及抗體稀釋和孵育等操作流程步驟的常見差異進(jìn)行了測試,并找出得到理想結(jié)果的方法,本次解析——裂解。
為什么專門解析裂解?那是因?yàn),在我們的技術(shù)支持幫助客戶解決大量技術(shù)難題的過程中,他們驚訝的發(fā)現(xiàn),WB不能曝出蛋白條帶或者蛋白條帶很弱,其大部分原因竟然都是蛋白樣品制備階段經(jīng)驗(yàn)不足或錯誤導(dǎo)致蛋白量不夠,現(xiàn)在就解析如何裂解才是最佳。
About Cell Signaling Technology (CST) , Inc.
Founded by research scientists in 1999, Cell Signaling Technology (CST) is a private,family-owned company headquartered in Danvers, Massachusetts with over 400 employees worldwide. Active in the field of applied systems biology research, particularly as it relates to cancer, CST understands the importance of using antibodies with high levels of specificity and lot-to-lot consistency. It’s why we produce all of our antibodies in house, and perform painstaking validations for multiple applications. And the same CST scientists who produce our antibodies also provide technical support for customers, helping them design experiments, troubleshoot, and achieve reliable results. We do this because that’s what we’d want if we were in the lab. Because, actually, we are.
www.cst-c.com.cn
技術(shù)熱線:4006-GreatQ/473287 技術(shù)支持:tech@cst-c.com.cn 產(chǎn)品訂購:info@cst-c.com.cn |
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- 附件 1 : 聽說大家都墻裂推薦這種方法.pdf
2015-12-29 16:46:40, 310.58 K
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