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三分餓的吧至尊木蟲 (著名寫手)
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[求助]
生化與分子生物學的一篇英文文獻摘要
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| A direct competitive enzyme-linked immunosorbent assay (ELISA) for triazophos was developed, which was based on the THHe monoclonal antibody (McAb) and a heterologous enzyme tracer (THBu-HRP). The influence of several physicochemical factors (temperature, time, pH, salt, detergent, and solvent) on the immunoassay was studied. For the standard curve, an I50 of 0.21 microg/L and a limit of detection (I20) of 0.02 microg/L was obtained in a high salt concentration buffer (0.05 M PBS, pH 6.0) with 0.05% BSA, which means an almost 3-fold improvement in the assay sensitivity in comparison with the nonoptimized conditions. The optimized ELISA has been used to quantify triazophos in water and soil samples spiked at different amounts. The excellent recoveries achieved confirmed the potential of the immunoassay for environmental monitoring of triazophos in waters and soils without purification steps. |
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287求調劑
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baoshanqiu
至尊木蟲 (著名寫手)
- 翻譯EPI: 204
- 應助: 103 (高中生)
- 金幣: 15293.5
- 紅花: 41
- 帖子: 1611
- 在線: 431.6小時
- 蟲號: 3856202
- 注冊: 2015-05-08
- 性別: GG
- 專業(yè): 胃腸道免疫相關疾病
【答案】應助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
三分餓的吧: 金幣+120, 翻譯EPI+1, ★★★★★最佳答案 2016-01-06 09:29:34
三分餓的吧: 金幣+120, 翻譯EPI+1, ★★★★★最佳答案 2016-01-06 09:29:34
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The influence of several physicochemical factors (temperature, time, pH, salt, detergent, and solvent) on the immunoassay was studied. For the standard curve, an I50 of 0.21 microg/L and a limit of detection (I20) of 0.02 microg/L was obtained in a high salt concentration buffer (0.05 M PBS, pH 6.0) with 0.05% BSA, which means an almost 3-fold improvement in the assay sensitivity in comparison with the nonoptimized conditions. The optimized ELISA has been used to quantify triazophos in water and soil samples spiked at different amounts. The excellent recoveries achieved confirmed the potential of the immunoassay for environmental monitoring of triazophos in waters and soils without purification steps. 采用THHe單克隆抗體(McAb)和一種非均相酶示蹤劑(THBu-HRP)建立了檢測三唑磷的直接競爭性酶聯(lián)免疫吸附試驗(ELISA)。探討了幾種理化因素（溫度、時間、pH、鹽、去垢劑和溶劑）對免疫檢測的影響。在含0.05% BSA高鹽濃度緩沖液(0.05 M PBS, pH 6.0)條件下，得到標準曲線的I50為0.21 microg/L，檢測限(I20) 為0.02 microg/L，意即較之于非優(yōu)化條件，其檢測靈敏度提高近3倍。優(yōu)化的ELISA已經用于定量檢測峰值不同的水和土壤樣品中得三唑磷。得到的良好回收率確認了運用免疫檢測法于環(huán)境監(jiān)測水和土壤中的三唑磷而無需純化過程的潛力。 |
2樓2016-01-05 23:19:12
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