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primer5.0安裝后無(wú)法輸入序列，怎么辦？注冊(cè)機(jī)怎么下載安裝？ 發(fā)自小木蟲Android客戶端 |
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小木蟲: 金幣+0.5, 給個(gè)紅包，謝謝回帖
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There are many computer programs available that can be used to design PCR primers, and their use is strongly recommeded, as these programs tend to incorporate several different parameters that the manual design of primers may fail to consider (i.e., Tm, intra- and/or intermolecular complementarity, mispriming libraries, etc.). We have had success using Primer3, which is available for use as an online tool (see http://frodo.wi.mit.edu/primer3/input.htm) as well as being freely available for download. References: Korbie D J, Mattick J S. Touchdown PCR for increased specificity and sensitivity in PCR amplification[J]. Nature Protocols, 2008, 3(9):1452-6. |
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