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無懈可擊wxkj新蟲 (小有名氣)
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[求助]
細(xì)胞生物學(xué)題目
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求助下面這道題的答案，用中文回答就可以，萬分感謝，急用，謝謝！ you are studying the secretory pathway in budding yeast using a combination of genetic, biochemical and cell biological techniques. (1)you are interested in performinga screen for temperature sensitive secretory mutants in budding yeast. what property of cells blocked in the secretory system will you exploit in this screen? how will you use this property to isolate mutants? (2)you isolate 5 classes of mutants in this screen. these classes are listed below, along with the location where proteins accumulate.describe what function is defective in each class, and briefly explain your answer. class A-Cytoplasm class B-Endoplasmic Reticulum class C-ER to Golgi Vessicles class D-Golgi class E-Secretory Vessicles (3)what protein might be mutated in Class A mutants? what protein might be mutanted in Class B mutants? (4)you isolate a mutant in which proteins that are normally targeted to the endosome instead accumulate in the Golgi. what protein might defective in these mutants? what other process might also be affected in these mutants? |
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