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lishuaiguang新蟲(chóng) (初入文壇)
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[求助]
蛋白質(zhì)不穩(wěn)定指數(shù)是什么？
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我在一篇文章上看到利用ExPASy在線蛋白質(zhì)分析軟件ProtParam分析HMGR基因編碼氨基酸的基本性質(zhì)，其氨基酸組成見(jiàn)表1。該蛋白亞基分子量約為60.5 kD，等電點(diǎn)pI=7.55。蛋白質(zhì)不穩(wěn)定指數(shù)(Instability Index)為44.31，屬于不穩(wěn)定蛋白質(zhì) 。 請(qǐng)問(wèn)蛋白質(zhì)不穩(wěn)定指數(shù)究竟是怎么定義的？ |
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大王的拖拉機(jī)
新蟲(chóng) (初入文壇)
- 應(yīng)助: 1 (幼兒園)
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- 專業(yè): 生物化學(xué)
【答案】應(yīng)助回帖
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Instability index (II) The instability index provides an estimate of the stability of your protein in a test tube. Statistical analysis of 12 unstable and 32 stable proteins has revealed [7] that there are certain dipeptides, the occurence of which is significantly different in the unstable proteins compared with those in the stable ones. The authors of this method have assigned a weight value of instability to each of the 400 different dipeptides (DIWV). Using these weight values it is possible to compute an instability index (II) which is defined as: i=L-1 II = (10/L) * Sum DIWV(xx[i+1]) i=1 where: L is the length of sequence DIWV(xx[i+1]) is the instability weight value for the dipeptide starting in position i. A protein whose instability index is smaller than 40 is predicted as stable, a value above 40 predicts that the protein may be unstable. |
2樓2017-04-15 14:25:17
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