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[求助]
Charged acrylamide copolymer gels as media for weak alignment
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求助。! 有沒有學(xué)長學(xué)姐做這個方向的,能不能提供一些相關(guān)的中文文獻(xiàn)。 以下是摘要 The use of mechanically strained acrylamide/acrylate copolymers is reported as a new alignment medium for biomacromolecules. Compared to uncharged, strained polyacrylamide gels, the negative charges of the acrylamide/ acrylate copolymer strongly alter the alignment tensor and lead to pronounced electroosmotic swelling. The swelling itself can be used to achieve anisotropic, mechanical strain. The method is demonstrated for the alignment of TipAS, a 17 kDa antibiotic resistance protein, as well as for human ubiquitin, where alignment tensors with an AZZ,NH of up to 60 Hz are achieved at a gel concentration of 2% (w/v). The alignment can be modulated by the variation of pH, ionic strength, and gel concentration. The high mechanical stability of the swollen gels makes it possible to obtain alignment at polymer concentrations of less than 1% (w/v). 好多專業(yè)名詞真是搞的頭都大了。 |
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據(jù)報道,機械應(yīng)變的丙烯酰胺/丙烯酸酯共聚物可作為一種新型排列介質(zhì)的生物大分子。跟不帶電荷的應(yīng)變聚丙烯酰胺凝膠相比,帶負(fù)電荷的應(yīng)變丙烯酰胺/丙烯酸酯共聚物可劇烈改變排列張量并導(dǎo)致明顯的電滲膨脹。這種電滲膨脹本身可用來實現(xiàn)各向異性應(yīng)變和機械應(yīng)變。該方法證明,在排列TipAS蛋白(一種蛋白分子量為17 kDa的抗生素抗性蛋白即人泛素蛋白),使其排列張量振幅(AZZ,NH)高于60赫茲,在2%(重量/體積)的凝膠濃度以實現(xiàn)。調(diào)變排列可通過調(diào)節(jié)pH變化、調(diào)節(jié)離子強度以及調(diào)節(jié)凝膠的濃度實現(xiàn)。電滲膨脹凝膠的高機械穩(wěn)定性使其在聚合物濃度小于1%(重量/體積)就能獲取排列。 (看完文章,明白了) |
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