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G號糖木蟲 (正式寫手)
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[求助]
請教pcr電泳方面問題 已有1人參與
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最近做了一般pcr,結(jié)果電泳圖感覺不行,想請教分析哈原因。如圖,基因擴(kuò)增片段大概1000bp,所以最下方是什么條帶?怎么避免呢 發(fā)自小木蟲Android客戶端 |
木蟲 (正式寫手)
木蟲 (正式寫手)
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The bands less than 100bp in size were primer dimers or RNA contaminations. When you extract DNA, treat the pellets with TE containing RNaseA at 37 degrees Celsius for 1 hour. When you amplify the target genes, use less primers and higher annealing temperature. By the way, set the positive and negetive controls on every experiment, which is really helpful to find out the carrier. 發(fā)自小木蟲IOS客戶端 |
木蟲 (著名寫手)
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