 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 723 | 回復(fù): 3 | ||
| 當(dāng)前只顯示滿足指定條件的回帖，點擊這里查看本話題的所有回帖 | ||
soloaaaa木蟲 (正式寫手)
|
[求助]
PET-HM是個什么質(zhì)粒啊，求助相關(guān)信息 已有1人參與
|
|
|
看文獻(xiàn)見到了個PET-HM質(zhì)粒，查不到相關(guān)的信息，求教。 文獻(xiàn)原文：The expression plasmid PET-HM was introduced into the T7-RNA-polymerasecontaining host E. coli BL21(DE3) (Studier et al., 1990). T7 RNA polymerase is used to drive the expression of DNA located downstream of the T7 promoter of the PET vector. Cells harboring PET-HM or PET-3a (control) were each grown under agitation at 37°C in Luria-Bertani medium containing 100 pg carbenicillidml, in the presence or absence of 100 pM lipoic acid, to an A,,, of 0.5 (about 2-2.5 h). Induction of the target prcitein was started by addition of isopropylthio-b-D-galactopyranclside to 0.4 mM. To monitor the induction of H protein 1 ml of culture was sampled at various times after induction. The cells were centrifuged (14000Xg, 1 min) and suspended in 100 p1 5DS/ PAGE sample buffer (Laemmli, 1970). After heating at 100°C for 3 min, 10 p1 of the sample (corresponding to 100 pl of culture) were subjected to |
回復(fù)此樓» 猜你喜歡
不合理蛙科研實驗之重金屬檢測：精準(zhǔn)篩查，守護(hù)健康與環(huán)境的防線
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗之“蛙測重金屬我背鍋三千”
已經(jīng)有0人回復(fù)
-
化學(xué)工程及工業(yè)化學(xué)論文潤色/翻譯怎么收費?
已經(jīng)有115人回復(fù)
-
不合理蛙科研實驗之“鼠逃三次我發(fā)三篇SCI”
已經(jīng)有0人回復(fù)
-
納米粒度分析
已經(jīng)有3人回復(fù)
-
林科院林化所招收材料與化工專業(yè)碩士，坐標(biāo)南京
已經(jīng)有0人回復(fù)
-
留學(xué)--博士招生
已經(jīng)有16人回復(fù)
-
化學(xué)工程，本211，求調(diào)劑，ab區(qū)不限
已經(jīng)有4人回復(fù)
-
邵陽學(xué)院食品與化學(xué)工程學(xué)院碩士調(diào)劑
已經(jīng)有0人回復(fù)
-
湖北師范大學(xué)招調(diào)劑啦
已經(jīng)有3人回復(fù)
soloaaaa
木蟲 (正式寫手)
- 應(yīng)助: 48 (小學(xué)生)
- 金幣: 2411.7
- 散金: 29
- 紅花: 1
- 帖子: 381
- 在線: 199.1小時
- 蟲號: 820780
- 注冊: 2009-08-03
- 性別: GG
- 專業(yè): 綠色有機化學(xué)
是我錯了，沒仔細(xì)看~ 謝謝啦~ 你也做這個嗎？ 
3樓2016-08-09 14:22:45
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★
感謝參與，應(yīng)助指數(shù) +1
soloaaaa: 金幣+5, ★★★★★最佳答案 2016-08-09 14:21:40
xn8008: 金幣+1, 歡迎發(fā)帖交流 2016-08-10 07:24:40
感謝參與，應(yīng)助指數(shù) +1
soloaaaa: 金幣+5, ★★★★★最佳答案 2016-08-09 14:21:40
xn8008: 金幣+1, 歡迎發(fā)帖交流 2016-08-10 07:24:40
|
樓主，你再把文獻(xiàn)好好看一看PET-HM應(yīng)該就是PET-3a裝了目的基因的表達(dá)載體，重新編了個名字。 原文是不是這個：“The Ndel-BamH1 fragment was subcloned into the expression vector PET-3a (Stratagene), digested with Ndel and BamHl , to yield the expression plasmid PET-HM.” |
2樓2016-08-09 13:53:45
4樓2016-08-09 14:41:10
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 抱歉 +4 | 田洪有 2026-03-30 | 4/200 |
|
|---|---|---|---|---|
|
[考研] 085602化工求調(diào)劑（331分） +8 | 111@127 2026-03-30 | 8/400 |
|
|
|
[考研]
 304材料求調(diào)劑
+5
|
鐘llll 2026-03-26 | 5/250 |
|
|
|
[考研] 289求調(diào)劑 +16 | 新時代材料 2026-03-27 | 16/800 |
|
|
|
[考研] 材料專碩 085600求調(diào)劑 +7 | BBQ233 2026-03-30 | 7/350 |
|
|
|
[考研] 化學(xué)0703 調(diào)劑 306分 一志愿211 +8 | 26要上岸 2026-03-28 | 8/400 |
|
|
|
[考研] 考研調(diào)劑 +5 | Sanmu-124 2026-03-26 | 5/250 |
|
|
|
[考研] 一志愿中南大學(xué)化學(xué)0703總分337求調(diào)劑 +6 | niko- 2026-03-27 | 6/300 |
|
|
|
[考研] 一志愿雙一流機械285分求調(diào)劑 +4 | 幸運的三木 2026-03-29 | 5/250 |
|
|
|
[考研] 327求調(diào)劑 +6 | 汲亦昊 2026-03-29 | 6/300 |
|
|
|
[考研] 349求調(diào)劑 +6 | 李木子啊哈哈 2026-03-25 | 6/300 |
|
|
|
[考研] 一志愿華理，數(shù)一英一285求A區(qū)調(diào)劑 +8 | AZMK 2026-03-25 | 12/600 |
|
|
|
[考研] 085602 307分 求調(diào)劑 +7 | 不知道叫什么！ 2026-03-26 | 7/350 |
|
|
|
[考研] 085405 考的11408求各位老師帶走 +3 | Qiu學(xué)ing 2026-03-28 | 3/150 |
|
|
|
[考研] 352分 化工與材料 +5 | 海納百川Ly 2026-03-27 | 5/250 |
|
|
|
[考研] 274求調(diào)劑 +17 | 顧九笙要謙虛 2026-03-24 | 23/1150 |
|
|
|
[考研] 求調(diào)劑 +3 | 劉柯@ 2026-03-24 | 4/200 |
|
|
|
[考研] 336材料求調(diào)劑 +7 | 陳瀅瑩 2026-03-26 | 9/450 |
|
|
|
[考研] 中國科學(xué)院深圳先進(jìn)技術(shù)研究院-光纖傳感課題組招生-中國科學(xué)院大學(xué)、深圳理工大學(xué)聯(lián)培 +5 | YangTyu1 2026-03-26 | 5/250 |
|
|
|
[考研] 一志愿武理085500機械專業(yè)總分300求調(diào)劑 +3 | an10101 2026-03-24 | 7/350 |
|