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soloaaaa木蟲 (正式寫手)
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[求助]
PET-HM是個什么質(zhì)粒啊，求助相關(guān)信息 已有1人參與
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看文獻(xiàn)見到了個PET-HM質(zhì)粒，查不到相關(guān)的信息，求教。 文獻(xiàn)原文：The expression plasmid PET-HM was introduced into the T7-RNA-polymerasecontaining host E. coli BL21(DE3) (Studier et al., 1990). T7 RNA polymerase is used to drive the expression of DNA located downstream of the T7 promoter of the PET vector. Cells harboring PET-HM or PET-3a (control) were each grown under agitation at 37°C in Luria-Bertani medium containing 100 pg carbenicillidml, in the presence or absence of 100 pM lipoic acid, to an A,,, of 0.5 (about 2-2.5 h). Induction of the target prcitein was started by addition of isopropylthio-b-D-galactopyranclside to 0.4 mM. To monitor the induction of H protein 1 ml of culture was sampled at various times after induction. The cells were centrifuged (14000Xg, 1 min) and suspended in 100 p1 5DS/ PAGE sample buffer (Laemmli, 1970). After heating at 100°C for 3 min, 10 p1 of the sample (corresponding to 100 pl of culture) were subjected to |
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湖北師范大學(xué)招調(diào)劑啦
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4樓2016-08-09 14:41:10
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★
感謝參與，應(yīng)助指數(shù) +1
soloaaaa: 金幣+5, ★★★★★最佳答案 2016-08-09 14:21:40
xn8008: 金幣+1, 歡迎發(fā)帖交流 2016-08-10 07:24:40
感謝參與，應(yīng)助指數(shù) +1
soloaaaa: 金幣+5, ★★★★★最佳答案 2016-08-09 14:21:40
xn8008: 金幣+1, 歡迎發(fā)帖交流 2016-08-10 07:24:40
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樓主，你再把文獻(xiàn)好好看一看PET-HM應(yīng)該就是PET-3a裝了目的基因的表達(dá)載體，重新編了個名字。 原文是不是這個：“The Ndel-BamH1 fragment was subcloned into the expression vector PET-3a (Stratagene), digested with Ndel and BamHl , to yield the expression plasmid PET-HM.” |
2樓2016-08-09 13:53:45
soloaaaa
木蟲 (正式寫手)
- 應(yīng)助: 48 (小學(xué)生)
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是我錯了，沒仔細(xì)看~ 謝謝啦~ 你也做這個嗎？ 3樓2016-08-09 14:22:45
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