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靜待花開(kāi)23金蟲(chóng) (正式寫(xiě)手)
嘻嘻哈哈
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[求助]
轉(zhuǎn)運(yùn)體體外實(shí)驗(yàn)(HEK293細(xì)胞的培養(yǎng)和轉(zhuǎn)染) 已有2人參與
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1.HEK293細(xì)胞和293T細(xì)胞有啥不一樣? 2.針對(duì)這兩種細(xì)胞做瞬時(shí)轉(zhuǎn)染,選用什么轉(zhuǎn)染方法?鈣轉(zhuǎn)法就夠了么?有哪些注意事項(xiàng)或者有相關(guān)的protocol么? 發(fā)自小木蟲(chóng)Android客戶端 |

至尊木蟲(chóng) (職業(yè)作家)
Felix Baumgartner Space Jump
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293: http://www.atcc.org/SearchCatalogs/longview.cfm?view=ce,913138,CRL-1573&text=CRL%2D1573&max=20 The 293 cell line is a permanent line of primary human embryonal kidney transformed by sheared human adenovirus type 5 (Ad 5) DNA. The cells express the transforming gene of adenovirus 5. 293T: http://www.atcc.org/SearchCatalogs/longview.cfm?view=ce,585784,CRL-11268&text=293&max=20 293T cells were cloned and co-transfected with the pBND and pZAP vectors to obtain the G418 resistant 293T/17 (ATCC CRL-11268) cell line. These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability. |
金蟲(chóng) (正式寫(xiě)手)
嘻嘻哈哈

至尊木蟲(chóng) (職業(yè)作家)
Felix Baumgartner Space Jump
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看看我寫(xiě)的帖子,能否幫到你。 http://www.gaoyang168.com/bbs/viewthread.php?tid=10511459 |
金蟲(chóng) (正式寫(xiě)手)
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禁蟲(chóng) (正式寫(xiě)手)
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