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靜待花開23金蟲 (正式寫手)
嘻嘻哈哈
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[求助]
轉運體體外實驗(HEK293細胞的培養(yǎng)和轉染) 已有2人參與
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1.HEK293細胞和293T細胞有啥不一樣? 2.針對這兩種細胞做瞬時轉染,選用什么轉染方法?鈣轉法就夠了么?有哪些注意事項或者有相關的protocol么? 發(fā)自小木蟲Android客戶端 |

至尊木蟲 (職業(yè)作家)
Felix Baumgartner Space Jump
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293: http://www.atcc.org/SearchCatalogs/longview.cfm?view=ce,913138,CRL-1573&text=CRL%2D1573&max=20 The 293 cell line is a permanent line of primary human embryonal kidney transformed by sheared human adenovirus type 5 (Ad 5) DNA. The cells express the transforming gene of adenovirus 5. 293T: http://www.atcc.org/SearchCatalogs/longview.cfm?view=ce,585784,CRL-11268&text=293&max=20 293T cells were cloned and co-transfected with the pBND and pZAP vectors to obtain the G418 resistant 293T/17 (ATCC CRL-11268) cell line. These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability. |
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至尊木蟲 (職業(yè)作家)
Felix Baumgartner Space Jump
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看看我寫的帖子,能否幫到你。 http://www.gaoyang168.com/bbs/viewthread.php?tid=10511459 |
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