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白玉浴血木蟲(chóng) (正式寫(xiě)手)
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[求助]
紫外可見(jiàn)吸收光譜，求大神幫忙解釋一下�。。。�
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To study and quantify the oxidation of thiols within the multilayers, 1 μm silica particles coated with five bilayers of PMASH/PVPON were used. In reduced form, the multilayers are unstable at pH 7 and rapidly disassemble when placed into a 10 mM solution of Ellman’s reagent in 1 M phosphate buffer, pH 7.5. The resulting solution has a characteristic absorption at 412 nm (Figure 2, spectrum 1), which is used to quantify the number of thiol groups by using a calibration curve obtained with standard solutions of glutathione. The addition of CaT to the particles with PMASH/PVPON multilayers at different pH (4–6) results in a decreased number of thiol groups, reflecting their oxidation into disulfide linkages. We found that the addition of 2 mole equivalents of CaT oxidized 90% of thiols within 1 min (Figure 2, spectrum 2), and a moderate excess of CaT (2 mM solution) converts the thiols into disulfides nearly quantitatively (Figure 2, spectrum 3). 請(qǐng)大神指教，這里的曲線1是一條校準(zhǔn)曲線，這是校準(zhǔn)的什么，這條曲線代表什么意義呢？曲線2和3又是怎么得來(lái)的雙硫鍵的氧化率呢？ 圖片1.png |
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